Detailed information of TA system TA230121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1008050..1008864
• Replicon: chromosome
• Accession: NZ_CP090141
• Organism: Salmonella enterica subsp. enterica serovar Goldcoast strain 5ASAL09

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: A0A8E5IG67
• Locus tag: LZK30_RS04805
• Protein ID: WP_000971651.1
• Coordinates: 1008050..1008577 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: M7S6I2
• Locus tag: LZK30_RS04810
• Protein ID: WP_000855694.1
• Coordinates: 1008574..1008864 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK30_RS04775 (1004324)	1004324..1004722	+	399	Protein_937	cytoplasmic protein	-
LZK30_RS04780 (1004913)	1004913..1005152	+	240	Protein_938	hypothetical protein	-
LZK30_RS04785 (1005309)	1005309..1005977	+	669	WP_000445914.1	hypothetical protein	-
LZK30_RS04790 (1006004)	1006004..1006498	+	495	WP_020899151.1	hypothetical protein	-
LZK30_RS04795 (1006670)	1006670..1007326	-	657	WP_023181885.1	protein-serine/threonine phosphatase	-
LZK30_RS04800 (1007559)	1007559..1007977	+	419	Protein_942	transposase	-
LZK30_RS04805 (1008050)	1008050..1008577	-	528	WP_000971651.1	GNAT family N-acetyltransferase	Toxin
LZK30_RS04810 (1008574)	1008574..1008864	-	291	WP_000855694.1	DUF1778 domain-containing protein	Antitoxin
LZK30_RS04815 (1009458)	1009458..1009784	+	327	WP_000393295.1	hypothetical protein	-
LZK30_RS04820 (1010057)	1010057..1010404	-	348	WP_001555786.1	DUF1493 family protein	-
LZK30_RS04825 (1010389)	1010389..1010838	-	450	WP_133322517.1	hypothetical protein	-
LZK30_RS04830 (1011270)	1011270..1011713	-	444	WP_000715096.1	SPI-1 type III secretion system invasion lipoprotein InvH	-
LZK30_RS04835 (1012170)	1012170..1012820	+	651	WP_001728903.1	type III secretion system transcriptional activator InvF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	invH / invF / invG / invE / invA / invB	1006670..1018133	11463
	-	flank	IS/Tn	-	-	1007801..1007977	176

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18970.77 Da
• Isoelectric Point: 9.3905

>T230121 WP_000971651.1 NZ_CP090141:c1008577-1008050 [Salmonella enterica subsp. enterica serovar Goldcoast]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGGYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Nucleotide

• Length: 528 bp

>T230121 NZ_CP124702:c4890204-4889287 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGTCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10664.50 Da
• Isoelectric Point: 6.3133

>AT230121 WP_000855694.1 NZ_CP090141:c1008864-1008574 [Salmonella enterica subsp. enterica serovar Goldcoast]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNVLPVAQKIVDAAERVYLTERDTQMIMEILDNPPAP
NEKLLAAAFALPDMKK

Nucleotide

• Length: 291 bp

>AT230121 NZ_CP124702:c4890968-4890210 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A3V8SJE7