Detailed information of TA system TA230077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 718..1298
• Replicon: plasmid pKP46_5
• Accession: NZ_CP090131
• Organism: Klebsiella pneumoniae strain KP46

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LWR79_RS27785
• Protein ID: WP_071177730.1
• Coordinates: 718..1032 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LWR79_RS27790
• Protein ID: WP_000093040.1
• Coordinates: 1020..1298 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWR79_RS27780 (LWR79_27780)	126..554	-	429	WP_001140599.1	hypothetical protein	-
LWR79_RS27785 (LWR79_27785)	718..1032	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LWR79_RS27790 (LWR79_27790)	1020..1298	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LWR79_RS27795 (LWR79_27795)	1473..1838	-	366	WP_072354022.1	TonB family protein	-
LWR79_RS27800 (LWR79_27800)	1835..2206	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LWR79_RS27805 (LWR79_27805)	2480..2725	-	246	WP_032440458.1	hypothetical protein	-
LWR79_RS27810 (LWR79_27810)	3052..4737	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
LWR79_RS27815 (LWR79_27815)	4747..5004	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
LWR79_RS27820 (LWR79_27820)	5089..5238	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T230077 WP_071177730.1 NZ_CP090131:c1032-718 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T230077 NZ_CP124680:c1565230-1564829 [Nocardioides sp. L-11A]
ATGAACCCGCGCCACCGTCACCTCCTCGACACGGACGTCGCCATCGAGCTGCTGCGCAAGCGGGACGTCGCGCTACGGGA
GGCGTGGCGGGCTGCGGGCCGGGTGGCCGCCTCGTCGGTCTCACTGTTCGAGCTGCGCTTCGGCGCTGCCCGCAGCGCCG
AGCCGGCTCGCAACGACCTCGCCGTCGACGAGCTGGCCGCCGCCGTCGACTTCCTCGACTTCGACGCCGAGGCGGCAGCA
CACGCCGGCGACATCCGCGCCGACCTCGCCCGCCTCGGCACGCCGATCGGCGCCTACGACGTCATGATCGCCGGGCACGC
CCGGTCCCGCGGGCTGATCGTCGTGACCCGCAACGAACGCGAGTTCCGGCGGGTCGACGGGCTCCGCGTCGAACGCTGGT
GA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT230077 WP_000093040.1 NZ_CP090131:c1298-1020 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT230077 NZ_CP124680:c1565460-1565227 [Nocardioides sp. L-11A]
ATGTCCGTGGTCACCAGGCTGTTCCTCTCGAACCGGACCCAGGCCGTGCGCCTTCCCAAGGCCGTCGCGTTCGACGCGTC
GGTGTCCGAGGTGGAGATCGACATCGTCGGCGATGCTCGGGTGATCCGTCCCGCCGGCTCGCGGGTCGACGCGTTCTGGA
GCAGCGACATGCGGGTCAGCGACGACTTCGCCCGCGAGCAGCCCGAAGCTCCCCAGGAGCGAGCCTGGGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1