Detailed information of TA system TA230076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 16895..17538
• Replicon: plasmid pKP46_4
• Accession: NZ_CP090130
• Organism: Klebsiella pneumoniae strain KP46

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: LWR79_RS27655
• Protein ID: WP_000754566.1
• Coordinates: 16895..17311 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: LWR79_RS27660
• Protein ID: WP_001261276.1
• Coordinates: 17308..17538 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWR79_RS27625 (LWR79_27625)	11997..13010	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
LWR79_RS27630 (LWR79_27630)	13213..13563	+	351	WP_000454193.1	DUF3330 domain-containing protein	-
LWR79_RS27635 (LWR79_27635)	13689..13871	+	183	Protein_18	recombinase family protein	-
LWR79_RS27640 (LWR79_27640)	13926..14630	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LWR79_RS27645 (LWR79_27645)	14693..15553	-	861	Protein_20	IS110 family transposase	-
LWR79_RS27650 (LWR79_27650)	15835..16691	-	857	Protein_21	IS3-like element ISEc15 family transposase	-
LWR79_RS27655 (LWR79_27655)	16895..17311	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
LWR79_RS27660 (LWR79_27660)	17308..17538	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LWR79_RS27665 (LWR79_27665)	18127..18420	+	294	WP_020956883.1	hypothetical protein	-
LWR79_RS27670 (LWR79_27670)	18484..19170	+	687	WP_020956882.1	hypothetical protein	-
LWR79_RS27675 (LWR79_27675)	19182..19958	+	777	WP_020956881.1	tyrosine-type recombinase/integrase	-
LWR79_RS27680 (LWR79_27680)	20016..20273	-	258	WP_000764642.1	hypothetical protein	-
LWR79_RS27685 (LWR79_27685)	20402..20506	-	105	WP_032409716.1	hypothetical protein	-
LWR79_RS27690 (LWR79_27690)	21036..21902	+	867	WP_004118283.1	replication initiation protein	-
LWR79_RS27695 (LWR79_27695)	22079..22348	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5	-	1..39224	39224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T230076 WP_000754566.1 NZ_CP090130:c17311-16895 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T230076 NZ_CP124670:c5332319-5332041 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT230076 WP_001261276.1 NZ_CP090130:c17538-17308 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT230076 NZ_CP124670:c5332029-5331724 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023