Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 93620..94202 Replicon plasmid pKP46_1_KPC
Accession NZ_CP090127
Organism Klebsiella pneumoniae strain KP46

Toxin (Protein)


Gene name PumA Uniprot ID E8PSG2
Locus tag LWR79_RS26585 Protein ID WP_013583078.1
Coordinates 93906..94202 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A223LLE5
Locus tag LWR79_RS26580 Protein ID WP_058998984.1
Coordinates 93620..93904 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWR79_RS26545 (LWR79_26545) 89132..89425 - 294 WP_058998996.1 TrbC/VirB2 family protein -
LWR79_RS26550 (LWR79_26550) 89443..90072 - 630 WP_058998994.1 lytic transglycosylase domain-containing protein -
LWR79_RS26555 (LWR79_26555) 90079..90276 - 198 WP_058998992.1 hypothetical protein -
LWR79_RS26560 (LWR79_26560) 90266..90760 - 495 WP_058998990.1 transcription termination/antitermination NusG family protein -
LWR79_RS26565 (LWR79_26565) 91152..91559 + 408 WP_072223386.1 helix-turn-helix transcriptional regulator -
LWR79_RS26570 (LWR79_26570) 91556..92725 - 1170 WP_058998988.1 MobP1 family relaxase -
LWR79_RS26575 (LWR79_26575) 92729..93316 - 588 WP_161400389.1 hypothetical protein -
LWR79_RS26580 (LWR79_26580) 93620..93904 - 285 WP_058998984.1 putative addiction module antidote protein Antitoxin
LWR79_RS26585 (LWR79_26585) 93906..94202 - 297 WP_013583078.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LWR79_RS26590 (LWR79_26590) 94541..94678 - 138 WP_161400388.1 hypothetical protein -
LWR79_RS26595 (LWR79_26595) 94725..94937 - 213 WP_058998981.1 hypothetical protein -
LWR79_RS26600 (LWR79_26600) 95343..95576 - 234 WP_072223385.1 hypothetical protein -
LWR79_RS26605 (LWR79_26605) 95566..95823 - 258 WP_058998978.1 hypothetical protein -
LWR79_RS26610 (LWR79_26610) 95820..96362 - 543 WP_058998977.1 hypothetical protein -
LWR79_RS26615 (LWR79_26615) 96362..96568 - 207 WP_058998975.1 hypothetical protein -
LWR79_RS26620 (LWR79_26620) 96580..96795 - 216 WP_058998973.1 hypothetical protein -
LWR79_RS26625 (LWR79_26625) 96837..97358 - 522 WP_058998971.1 J domain-containing protein -
LWR79_RS26630 (LWR79_26630) 97471..97692 + 222 WP_058998969.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..103807 103807


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-89)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11290.10 Da        Isoelectric Point: 10.3166

>T230073 WP_013583078.1 NZ_CP090127:c94202-93906 [Klebsiella pneumoniae]
MIEIKRTPEVEKWLRSLKDRTAKAKILMRLERMEDGNFGDVEPIGDGLSELRIHQGKGYRVYFGNKNNQIILLLCGGDKS
TQQADIRKAKQLAKQWGF

Download         Length: 297 bp

>T230073 NZ_CP124670:1820073-1820660 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGTATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGTGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10557.97 Da        Isoelectric Point: 4.7254

>AT230073 WP_058998984.1 NZ_CP090127:c93904-93620 [Klebsiella pneumoniae]
MKEELKKFDVVEFLEDEEDIQEYLNAAIEEDDTKYLFKALGNIARARNISQLSKEVGMSREGIYKALSGEGNPSFQTVSK
ITKALGLKLHFTGA

Download         Length: 285 bp

>AT230073 NZ_CP124670:1820727-1821581 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGCTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGACAGCCTGGAAACCCAGACCCGCTGGCGTGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCATCCTTGACGATCTACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A223LLP6

Antitoxin

Source ID Structure
AlphaFold DB A0A223LLE5

References