230072

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5296587..5297212 Replicon chromosome
Accession NZ_CP090126
Organism Klebsiella pneumoniae strain KP46

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LWR79_RS25675 Protein ID WP_002882817.1
Coordinates 5296587..5296970 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LWR79_RS25680 Protein ID WP_004150355.1
Coordinates 5296970..5297212 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWR79_RS25660 (5293953) 5293953..5294855 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LWR79_RS25665 (5294852) 5294852..5295487 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LWR79_RS25670 (5295484) 5295484..5296413 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LWR79_RS25675 (5296587) 5296587..5296970 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LWR79_RS25680 (5296970) 5296970..5297212 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LWR79_RS25685 (5297417) 5297417..5298334 + 918 WP_004181612.1 alpha/beta hydrolase -
LWR79_RS25690 (5298348) 5298348..5299289 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LWR79_RS25695 (5299334) 5299334..5299771 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LWR79_RS25700 (5299768) 5299768..5300628 - 861 WP_004146232.1 virulence factor BrkB family protein -
LWR79_RS25705 (5300622) 5300622..5301221 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230072 WP_002882817.1 NZ_CP090126:c5296970-5296587 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230072 NZ_CP124670:c141822-141541 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAAGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230072 WP_004150355.1 NZ_CP090126:c5297212-5296970 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230072 NZ_CP124670:c142046-141819 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References