Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 699585..700252 Replicon plasmid pRL1
Accession NZ_CP090103
Organism Rhizobium leguminosarum strain Tp73_4

Toxin (Protein)


Gene name vapC Uniprot ID Q1M497
Locus tag LZK79_RS35835 Protein ID WP_011649135.1
Coordinates 699585..699995 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q1M496
Locus tag LZK79_RS35840 Protein ID WP_011649136.1
Coordinates 699992..700252 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK79_RS35820 (LZK79_35830) 695441..696262 + 822 WP_233939112.1 3-methyl-2-oxobutanoate hydroxymethyltransferase -
LZK79_RS35825 (LZK79_35835) 696284..697198 - 915 WP_011649133.1 hydrogen peroxide-inducible genes activator -
LZK79_RS35830 (LZK79_35840) 697342..699522 + 2181 WP_028742375.1 catalase/peroxidase HPI -
LZK79_RS35835 (LZK79_35845) 699585..699995 - 411 WP_011649135.1 type II toxin-antitoxin system VapC family toxin Toxin
LZK79_RS35840 (LZK79_35850) 699992..700252 - 261 WP_011649136.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LZK79_RS35845 (LZK79_35855) 700388..700947 - 560 Protein_644 DUF3299 domain-containing protein -
LZK79_RS35850 (LZK79_35860) 701055..702023 - 969 WP_028742373.1 zinc-dependent alcohol dehydrogenase family protein -
LZK79_RS35855 (LZK79_35865) 702077..702493 + 417 WP_164580700.1 thioesterase family protein -
LZK79_RS35860 (LZK79_35870) 702523..704052 + 1530 WP_164580701.1 AMP-binding protein -
LZK79_RS35865 (LZK79_35875) 704030..705235 + 1206 WP_028742370.1 thiolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..825442 825442


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(8-45)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14781.89 Da        Isoelectric Point: 4.9105

>T230048 WP_011649135.1 NZ_CP090103:c699995-699585 [Rhizobium leguminosarum]
VITHLIDTNAVIALIGRKSETLLKRVIDSDEGSIGLSTVVMHELYYGAYKSTKISYNLETLRLFVADFSVVGFEQEDALA
AGEIRAALAAKGTPIGPYDVLIAAQARTRDLVLVTNNVGEFGRVDGLRVEDWTVGR

Download         Length: 411 bp

>T230048 NZ_CP124666:1911611-1912198 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGACGTTGCCGCTCGCTGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGTGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9948.12 Da        Isoelectric Point: 5.1532

>AT230048 WP_011649136.1 NZ_CP090103:c700252-699992 [Rhizobium leguminosarum]
MPQYARVFQSGNSQAVRLPKEFRFDVDQVEVTREGDAVILRPRADRGMRWASLHSALERGLSDDFMAEGREQPEAQERPD
LQNLFK

Download         Length: 261 bp

>AT230048 NZ_CP124666:1912265-1913119 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGCTCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGAGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGAGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q1M497

Antitoxin

Source ID Structure
AlphaFold DB Q1M496

References