Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 361673..362349 Replicon plasmid pRL1
Accession NZ_CP090103
Organism Rhizobium leguminosarum strain Tp73_4

Toxin (Protein)


Gene name vapC Uniprot ID Q1M527
Locus tag LZK79_RS34315 Protein ID WP_011648871.1
Coordinates 361924..362349 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q1M528
Locus tag LZK79_RS34310 Protein ID WP_011648870.1
Coordinates 361673..361927 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK79_RS34280 (LZK79_34290) 356683..357525 - 843 WP_233939186.1 crotonase/enoyl-CoA hydratase family protein -
LZK79_RS34285 (LZK79_34295) 357614..358204 + 591 WP_225510491.1 TetR/AcrR family transcriptional regulator -
LZK79_RS34290 (LZK79_34300) 358461..359300 + 840 WP_011648866.1 transporter substrate-binding domain-containing protein -
LZK79_RS34295 (LZK79_34305) 359418..360080 + 663 WP_028742556.1 amino acid ABC transporter permease -
LZK79_RS34300 (LZK79_34310) 360086..360745 + 660 WP_011648868.1 amino acid ABC transporter permease -
LZK79_RS34305 (LZK79_34315) 360758..361528 + 771 WP_233939187.1 amino acid ABC transporter ATP-binding protein -
LZK79_RS34310 (LZK79_34320) 361673..361927 + 255 WP_011648870.1 plasmid stabilization protein Antitoxin
LZK79_RS34315 (LZK79_34325) 361924..362349 + 426 WP_011648871.1 type II toxin-antitoxin system VapC family toxin Toxin
LZK79_RS34320 (LZK79_34330) 362542..363303 + 762 WP_011648872.1 GntR family transcriptional regulator -
LZK79_RS34325 (LZK79_34335) 363297..364082 + 786 WP_012755579.1 transporter substrate-binding domain-containing protein -
LZK79_RS34330 (LZK79_34340) 364160..364831 + 672 WP_011648874.1 amino acid ABC transporter permease -
LZK79_RS34335 (LZK79_34345) 364828..365487 + 660 WP_011648875.1 amino acid ABC transporter permease -
LZK79_RS34340 (LZK79_34350) 365465..366190 + 726 WP_011648876.1 amino acid ABC transporter ATP-binding protein -
LZK79_RS34345 (LZK79_34355) 366238..366609 - 372 WP_233939188.1 type II toxin-antitoxin system RelE/ParE family toxin -
LZK79_RS34350 (LZK79_34360) 366609..366866 - 258 WP_011648878.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..825442 825442


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-125)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 14976.18 Da        Isoelectric Point: 4.8838

>T230046 WP_011648871.1 NZ_CP090103:361924-362349 [Rhizobium leguminosarum]
MIILDTNVVSEAMKPTPDEAVKFWLDEQAAETLFLSSVTIAELMFGIGALPAGKRRERLSDALDGLMELFESRILAFDIT
AARHYADLAVKARAAGRGFPTPDGYIAAIAASKGFAVATRDTSAFNAAGVKVINPWAASTA

Download         Length: 426 bp

>T230046 NZ_CP124666:c143129-142848 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9361.70 Da        Isoelectric Point: 10.6531

>AT230046 WP_011648870.1 NZ_CP090103:361673-361927 [Rhizobium leguminosarum]
MPAVTIRNLSEATHRALKVRAAHHGRSAEAEMREILEAAVRPETRLRLGSALAERSRRLGLTNEDFAVLDQARDTLPAKP
MSFE

Download         Length: 255 bp

>AT230046 NZ_CP124666:c143353-143126 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q1M527

Antitoxin

Source ID Structure
AlphaFold DB A0A7Y3S1G5

References