Detailed information of TA system TA230043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 39065..40583
• Replicon: plasmid pRLTp73_3
• Accession: NZ_CP090101
• Organism: Rhizobium leguminosarum strain Tp73_4

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LZK79_RS27800
• Protein ID: WP_233938941.1
• Coordinates: 39675..40583 (+)
• Length: 303 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: C6BB39
• Locus tag: LZK79_RS27795
• Protein ID: WP_003555692.1
• Coordinates: 39065..39685 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK79_RS27780 (LZK79_27790)	34823..35131	-	309	WP_233938874.1	mobilization protein	-
LZK79_RS27785 (LZK79_27795)	35419..38706	+	3288	WP_233938875.1	Ti-type conjugative transfer relaxase TraA	-
LZK79_RS27790 (LZK79_27800)	38735..38972	-	238	Protein_26	chromosome partitioning protein ParB	-
LZK79_RS27795 (LZK79_27805)	39065..39685	+	621	WP_003555692.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LZK79_RS27800 (LZK79_27810)	39675..40583	+	909	WP_233938941.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LZK79_RS27805 (LZK79_27815)	40613..40903	-	291	Protein_29	type II toxin-antitoxin system RelE/ParE family toxin	-
LZK79_RS27810 (LZK79_27820)	40903..41184	-	282	WP_233938876.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LZK79_RS27815 (LZK79_27825)	41404..42396	+	993	WP_168311855.1	LLM class flavin-dependent oxidoreductase	-
LZK79_RS27820 (LZK79_27830)	42422..43771	+	1350	WP_233938877.1	LLM class flavin-dependent oxidoreductase	-
LZK79_RS27825 (LZK79_27835)	43789..44988	+	1200	WP_233938878.1	M20 aminoacylase family protein	-
LZK79_RS27830 (LZK79_27840)	44985..45542	+	558	WP_233938879.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..371703	371703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-272)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (13-56)

Sequences

Toxin

Protein

• Length: 303 a.a.
• Molecular weight: 33834.82 Da
• Isoelectric Point: 6.2944

>T230043 WP_233938941.1 NZ_CP090101:39675-40583 [Rhizobium leguminosarum]
MARELKKNVGASVRARLLDRSRAERTDFQILLTRYALERLLYRLSLSEHRDRFILKGAMLFVTWVADPFRPTRDLDLLGY
GENSPKAIAETFHAICTQPVDADGVVFDVDALAAAPIREEVEYGGVRVRTTATIGGARIPIQVDIGFGDAVTPGPVDIEY
PSLLDAPVPRLRAYPVATVVAEKFEALVSLGMANSRLKDFYDLWLIAETFELERSTLAEAVRQTFARRGTSIPTDRPTGL
SEAYAEVWDRQWRAFLGRERMAAAPPALGTVVADLGRFLLPLTQPVEGEWRWKPHEGWIQLK

Nucleotide

• Length: 909 bp

>T230043 NZ_CP124665:3580133-3580711 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTTTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGTTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 23103.87 Da
• Isoelectric Point: 9.8453

>AT230043 WP_003555692.1 NZ_CP090101:39065-39685 [Rhizobium leguminosarum]
MTFTTAQRLRALDLLKARGMLRLKDFVAEGIGPETLARLVREEAVVRPARGLYQLPDTQAEAAHALAEAAVLVPKGVVCL
TSALQYHELTLQMPSAVWMAIDRTAWRPKIDYPPIKFVRFKGSALTEGVERHRIEGVEVPITNPARSIVDCFRYRAKVGL
DVAMEGLREGLRRRKTTSDDLWTYAKEARIWSIMRPYVEATVADGA

Nucleotide

• Length: 621 bp

>AT230043 NZ_CP124665:3579491-3580045 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAAGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAAGCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCCCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A198YJQ3