Detailed information of TA system TA230041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 1213315..1214191
• Replicon: plasmid pNg7g1
• Accession: NZ_CP090096
• Organism: Neorhizobium galegae strain 7g

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LZK81_RS28465
• Protein ID: WP_233957349.1
• Coordinates: 1213315..1213830 (-)
• Length: 172 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LZK81_RS28470
• Protein ID: WP_233957350.1
• Coordinates: 1213823..1214191 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK81_RS28435 (LZK81_28440)	1208582..1208917	-	336	WP_037084912.1	hypothetical protein	-
LZK81_RS28440 (LZK81_28445)	1208904..1209269	-	366	WP_046605982.1	Rieske 2Fe-2S domain-containing protein	-
LZK81_RS28445 (LZK81_28450)	1209364..1210170	+	807	WP_233957346.1	IclR family transcriptional regulator	-
LZK81_RS28450 (LZK81_28455)	1210269..1210748	+	480	WP_233957347.1	MarR family transcriptional regulator	-
LZK81_RS28455 (LZK81_28460)	1211020..1211421	-	402	WP_046605980.1	response regulator	-
LZK81_RS28460 (LZK81_28465)	1211433..1213139	-	1707	WP_233957348.1	ATP-binding protein	-
LZK81_RS28465 (LZK81_28470)	1213315..1213830	-	516	WP_233957349.1	RES domain-containing protein	Toxin
LZK81_RS28470 (LZK81_28475)	1213823..1214191	-	369	WP_233957350.1	DUF2384 domain-containing protein	Antitoxin
LZK81_RS28475 (LZK81_28480)	1214267..1214911	-	645	WP_233957351.1	SMC-Scp complex subunit ScpB	-
LZK81_RS28480 (LZK81_28485)	1214914..1215864	-	951	WP_233957352.1	DUF1403 family protein	-
LZK81_RS28485 (LZK81_28490)	1216175..1217704	+	1530	WP_233957353.1	carboxypeptidase	-
LZK81_RS28490 (LZK81_28495)	1217826..1218548	+	723	WP_233957354.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..1254988	1254988

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (11-164)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (70-119)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 18738.25 Da
• Isoelectric Point: 6.3263

>T230041 WP_233957349.1 NZ_CP090096:c1213830-1213315 [Neorhizobium galegae]
MPELASESGILWRAYVPRWAHAPLSGDGAARFGGRWNPVGAATIYAARELSTAWAEYNQGFVQHPALIAQLRLDGARLAD
LTNPEVLVGLGVDETIHRCEWRADLDAGRIPSTHLLAERLLHDGCDGLIYPSFMSPGGTCVTLWRWNGKDQPKLTVTDPD
GRLPKSPASWL

Nucleotide

• Length: 516 bp

>T230041 NZ_CP124665:1642433-1643302 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 12985.08 Da
• Isoelectric Point: 10.1736

>AT230041 WP_233957350.1 NZ_CP090096:c1214191-1213823 [Neorhizobium galegae]
MAHVFEIGAAAFREPQSALLSARRVSNLLGLTHAELAKLIGVARNTLTAKSGARKVDLALSPVVRILAMASEMAGDENRA
AIWFKHQPIPGWAGKTAYDLVGEGKADKVLAYLEAVRSGVYA

Nucleotide

• Length: 369 bp

>AT230041 NZ_CP124665:1643295-1644128 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA