Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 917531..918116 Replicon plasmid pNg7g1
Accession NZ_CP090096
Organism Neorhizobium galegae strain 7g

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag LZK81_RS27040 Protein ID WP_233957043.1
Coordinates 917531..917818 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag LZK81_RS27045 Protein ID WP_046604704.1
Coordinates 917820..918116 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK81_RS27010 (LZK81_27015) 912746..913633 - 888 WP_233957035.1 hypothetical protein -
LZK81_RS27015 (LZK81_27020) 913879..914673 + 795 WP_233957037.1 molybdate ABC transporter substrate-binding protein -
LZK81_RS27020 (LZK81_27025) 914973..915203 + 231 WP_233957040.1 hypothetical protein -
LZK81_RS27025 (LZK81_27030) 915289..915516 + 228 WP_046604708.1 hypothetical protein -
LZK81_RS27030 (LZK81_27035) 915729..916430 + 702 WP_046604707.1 molybdate ABC transporter permease subunit -
LZK81_RS27035 (LZK81_27040) 916427..917488 + 1062 WP_233957041.1 molybdenum ABC transporter ATP-binding protein -
LZK81_RS27040 (LZK81_27045) 917531..917818 + 288 WP_233957043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LZK81_RS27045 (LZK81_27050) 917820..918116 + 297 WP_046604704.1 putative addiction module antidote protein Antitoxin
LZK81_RS27050 (LZK81_27055) 918299..919645 + 1347 WP_233957045.1 extracellular solute-binding protein -
LZK81_RS27055 (LZK81_27060) 919778..921079 + 1302 WP_233957047.1 sugar ABC transporter permease -
LZK81_RS27060 (LZK81_27065) 921082..921939 + 858 WP_233957049.1 carbohydrate ABC transporter permease -
LZK81_RS27065 (LZK81_27070) 921939..922994 + 1056 WP_233957051.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - htpB 1..1254988 1254988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-89)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10807.59 Da        Isoelectric Point: 10.1760

>T230040 WP_233957043.1 NZ_CP090096:917531-917818 [Neorhizobium galegae]
MVEVRRTAAFSKWFNSLKDIRAQARIQTRIDRLGLGLLGDAKFFDGIGELRIDYGPGYRLYFVKRGSEIILLLCGGDKSA
QRKDIQQAIEMAKEI

Download         Length: 288 bp

>T230040 NZ_CP124665:c143129-142848 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10263.79 Da        Isoelectric Point: 4.4501

>AT230040 WP_046604704.1 NZ_CP090096:917820-918116 [Neorhizobium galegae]
MAIETTKWDPTEFLTSEEAIAAYLEAAFEDGDPAVIAVVLGNIARAKGMTNVAKEAGITREALYKALSDKGDPKLSTLLG
VLKALGLRVTVVSANEAA

Download         Length: 297 bp

>AT230040 NZ_CP124665:c143353-143126 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References