Detailed information of TA system TA230038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 2909528..2910014
• Replicon: chromosome
• Accession: NZ_CP090095
• Organism: Neorhizobium galegae strain 7g

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LZK81_RS14450
• Protein ID: WP_233953712.1
• Coordinates: 2909751..2910014 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LZK81_RS14445
• Protein ID: WP_233953711.1
• Coordinates: 2909528..2909764 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK81_RS14425 (LZK81_14425)	2904841..2905794	+	954	WP_046609374.1	metallophosphoesterase	-
LZK81_RS14430 (LZK81_14430)	2905893..2906378	+	486	WP_046603823.1	RNA polymerase sigma factor	-
LZK81_RS14435 (LZK81_14435)	2906381..2907226	+	846	WP_233953709.1	anti-sigma factor	-
LZK81_RS14440 (LZK81_14440)	2907492..2909201	-	1710	WP_233953710.1	2-isopropylmalate synthase	-
LZK81_RS14445 (LZK81_14445)	2909528..2909764	-	237	WP_233953711.1	BrnA antitoxin family protein	Antitoxin
LZK81_RS14450 (LZK81_14450)	2909751..2910014	-	264	WP_233953712.1	BrnT family toxin	Toxin
LZK81_RS14455 (LZK81_14455)	2910053..2911231	-	1179	WP_046609229.1	benzoate/H(+) symporter BenE family transporter	-
LZK81_RS14460 (LZK81_14460)	2911515..2912459	+	945	WP_233953713.1	sensor domain-containing diguanylate cyclase	-
LZK81_RS14465 (LZK81_14465)	2912637..2913608	+	972	WP_233953714.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10254.85 Da
• Isoelectric Point: 5.2356

>T230038 WP_233953712.1 NZ_CP090095:c2910014-2909751 [Neorhizobium galegae]
MLFEFDPDKSAANKDKHGIDLVEAQALWADEKLFIMPAKDDREPRLMVIGEMDEKLWSAICVYRGERIRIISVRRSRKLE
VEVYESR

Nucleotide

• Length: 264 bp

>T230038 NZ_CP124664:c4855476-4855129 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9016.29 Da
• Isoelectric Point: 6.3533

>AT230038 WP_233953711.1 NZ_CP090095:c2909764-2909528 [Neorhizobium galegae]
MKAGKLDEIIDKGEEDVLEYFDVSKGYRPNLEPKRVNIDFPTWVVNGLDQEAQRLGVTRQSLVKLWIAERLEASRRGK

Nucleotide

• Length: 237 bp

>AT230038 NZ_CP124664:c4855737-4855486 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA