Detailed information of TA system TA230037

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2886791..2887393
• Replicon: chromosome
• Accession: NZ_CP090095
• Organism: Neorhizobium galegae strain 7g

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LZK81_RS14325
• Protein ID: WP_233953697.1
• Coordinates: 2886791..2887087 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LZK81_RS14330
• Protein ID: WP_233953698.1
• Coordinates: 2887091..2887393 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK81_RS14300 (LZK81_14300)	2882736..2883065	-	330	WP_052753492.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LZK81_RS14305 (LZK81_14305)	2883062..2883331	-	270	WP_046603843.1	transcriptional regulator	-
LZK81_RS14310 (LZK81_14310)	2883462..2885318	-	1857	WP_233953694.1	ABC transporter ATP-binding protein	-
LZK81_RS14315 (LZK81_14315)	2885699..2886178	+	480	WP_233953695.1	tRNA (cytidine(34)-2'-O)-methyltransferase	-
LZK81_RS14320 (LZK81_14320)	2886247..2886729	+	483	WP_233953696.1	redox-sensitive transcriptional activator SoxR	-
LZK81_RS14325 (LZK81_14325)	2886791..2887087	+	297	WP_233953697.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZK81_RS14330 (LZK81_14330)	2887091..2887393	+	303	WP_233953698.1	putative addiction module antidote protein	Antitoxin
LZK81_RS14335 (LZK81_14335)	2887554..2887982	+	429	WP_233953699.1	hypothetical protein	-
LZK81_RS14340 (LZK81_14340)	2887992..2888249	-	258	WP_046603838.1	hypothetical protein	-
LZK81_RS14345 (LZK81_14345)	2888425..2889339	+	915	WP_233953700.1	oxygen-dependent coproporphyrinogen oxidase	-
LZK81_RS14350 (LZK81_14350)	2889467..2889733	+	267	WP_046609245.1	hypothetical protein	-
LZK81_RS14355 (LZK81_14355)	2889837..2890028	+	192	WP_046603835.1	DUF1059 domain-containing protein	-
LZK81_RS14360 (LZK81_14360)	2890112..2891374	-	1263	WP_233953701.1	CCA tRNA nucleotidyltransferase	-
LZK81_RS14365 (LZK81_14365)	2891371..2892006	-	636	WP_046603833.1	CoA pyrophosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (4-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11207.72 Da
• Isoelectric Point: 8.0200

>T230037 WP_233953697.1 NZ_CP090095:2886791-2887087 [Neorhizobium galegae]
MFTVYSTKVFDDWVDDLRDRHAVTRIFGRIARAEEGNLGDVKPVGEGVSEMRIHYGPGYRIYFSRRGDIVVLLLCGGDKS
SQTRDVAEAKRLAKEWKE

Nucleotide

• Length: 297 bp

>T230037 NZ_CP124664:c4756310-4755945 [Pseudomonas aeruginosa]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGAGGGCCATCCTTGGGGCACCTCCACAGCAGCGGCATCAATGGCTCCCGCCATG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGATCCCAGACGCTCCGCG
ATACTGCTGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCTATGA
CGAACACTTGCACCAGCTCCGTGAGGGGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10839.58 Da
• Isoelectric Point: 4.6286

>AT230037 WP_233953698.1 NZ_CP090095:2887091-2887393 [Neorhizobium galegae]
MALEIRRFDIAEHLNSDEDIQQFMIAAFEDGDPAFIAKCLGIVARAQNMSKLARDVGMSRAALYKALSGEGNPEFATVLK
VMKALGLELTPVLVKAEDAA

Nucleotide

• Length: 303 bp

>AT230037 NZ_CP124664:c4755952-4755629 [Pseudomonas aeruginosa]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGGCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCACGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATCGCACGCTTCCCGGATGGCGCGGTGAAAATCAGCAACTTCGCTGATATCGAAGAGGCGGGCGC
CTAA