Detailed information of TA system TA230033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 1125887..1126473
• Replicon: chromosome
• Accession: NZ_CP090095
• Organism: Neorhizobium galegae strain 7g

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LZK81_RS05480
• Protein ID: WP_046607137.1
• Coordinates: 1126096..1126473 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0T7F8Q4
• Locus tag: LZK81_RS05475
• Protein ID: WP_046607262.1
• Coordinates: 1125887..1126102 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK81_RS05450 (LZK81_05450)	1120916..1121953	+	1038	WP_233955427.1	sulfate/molybdate ABC transporter ATP-binding protein	-
LZK81_RS05455 (LZK81_05455)	1122187..1123506	+	1320	WP_233955428.1	phosphatidylglycerol lysyltransferase domain-containing protein	-
LZK81_RS05460 (LZK81_05460)	1123503..1124864	+	1362	WP_233955429.1	AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase	-
LZK81_RS05465 (LZK81_05465)	1125006..1125350	+	345	WP_233956480.1	DUF6152 family protein	-
LZK81_RS05470 (LZK81_05470)	1125358..1125828	+	471	WP_233955430.1	DUF2214 domain-containing protein	-
LZK81_RS05475 (LZK81_05475)	1125887..1126102	+	216	WP_046607262.1	CopG family transcriptional regulator	Antitoxin
LZK81_RS05480 (LZK81_05480)	1126096..1126473	+	378	WP_046607137.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZK81_RS05485 (LZK81_05485)	1126474..1128126	-	1653	WP_233955431.1	choline dehydrogenase	-
LZK81_RS05490 (LZK81_05490)	1128370..1129833	-	1464	WP_233955432.1	betaine-aldehyde dehydrogenase	-
LZK81_RS05495 (LZK81_05495)	1129835..1131358	-	1524	WP_233955433.1	choline-sulfatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-108)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13981.08 Da
• Isoelectric Point: 5.5742

>T230033 WP_046607137.1 NZ_CP090095:1126096-1126473 [Neorhizobium galegae]
MVSDSAVLDTNIIVDYLNSVSEGREEFERYENPSISIVTWMEVLVGVPPHLAAATREFLNALNIIALDDAVAERAVSLRQ
KHKMKLPDAIIWASADTHSMLLVTRNTKDFPANLPGIRVPYVLRH

Nucleotide

• Length: 378 bp

>T230033 NZ_CP124664:1211938-1212252 [Pseudomonas aeruginosa]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTGGTAGACGACGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCTGACGCCGGAGTGGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCAGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCATTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGCCTAT
GACAAGGCATCGCAGGAAGATCTGACTGCCGATCAGAAAAAGATGCTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8267.33 Da
• Isoelectric Point: 4.7962

>AT230033 WP_046607262.1 NZ_CP090095:1125887-1126102 [Neorhizobium galegae]
MQTVVELPDAEVEALDRLAQKENISRSVLIERAVHDFLQKSDKQKRAEAFGLWGNRIVDGLDFQQKVRDEW

Nucleotide

• Length: 216 bp

>AT230033 NZ_CP124664:1212255-1212560 [Pseudomonas aeruginosa]
ATGGACAAAGAACTCTTCGCTGAACTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGTGGCGAACGTCAACCTTC
CCGCGAGTTCCACGTCGACGCGGTGAAGGTCAAAGAAATCCGACGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGAA
AGATCGATGTTGCTGTTGGAACCCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCTCGCGCCCTGCTC
CGGGCCATTGACAATGATCCGGTTCATGTTCTGGCTGCGCTGAATGGTGGCCCGCGAGAAAGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0T7F8Q4