Detailed information of TA system TA230032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1045899..1046458
• Replicon: chromosome
• Accession: NZ_CP090095
• Organism: Neorhizobium galegae strain 7g

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LZK81_RS05035
• Protein ID: WP_046625071.1
• Coordinates: 1046210..1046458 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LZK81_RS05030
• Protein ID: WP_046610988.1
• Coordinates: 1045899..1046213 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK81_RS05010 (LZK81_05010)	1041364..1042596	+	1233	WP_233955382.1	AGE family epimerase/isomerase	-
LZK81_RS05015 (LZK81_05015)	1042601..1043668	+	1068	WP_233955383.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
LZK81_RS05020 (LZK81_05020)	1043690..1044865	+	1176	WP_046603453.1	mannonate dehydratase	-
LZK81_RS05025 (LZK81_05025)	1044891..1045892	+	1002	WP_046603454.1	Gfo/Idh/MocA family oxidoreductase	-
LZK81_RS05030 (LZK81_05030)	1045899..1046213	-	315	WP_046610988.1	BrnA antitoxin family protein	Antitoxin
LZK81_RS05035 (LZK81_05035)	1046210..1046458	-	249	WP_046625071.1	BrnT family toxin	Toxin
LZK81_RS05040 (LZK81_05040)	1046548..1047648	-	1101	WP_233955384.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LZK81_RS05045 (LZK81_05045)	1047661..1048758	-	1098	WP_233955385.1	chorismate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-102)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9242.86 Da
• Isoelectric Point: 10.1451

>T230032 WP_046625071.1 NZ_CP090095:c1046458-1046210 [Neorhizobium galegae]
MKIIWDETKRIVNLEKHGLDFADLYFEFFATAVTTPAKKARSKAVGRLKDGTIVVIFLALGSEAISVISMRPARKDERSM
IE

Nucleotide

• Length: 249 bp

>T230032 NZ_CP124664:c144430-144149 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11871.76 Da
• Isoelectric Point: 9.9713

>AT230032 WP_046610988.1 NZ_CP090095:c1046213-1045899 [Neorhizobium galegae]
MNIKHEKQKEFRPGRGYTKEDWDAVDSPPLTAEEMASMRPFREVFPEMAAKMEQAIAARGRPKLEAPKVAVTLRLDPDVL
EKFKATGKDWRAKMTEELRKAAGL

Nucleotide

• Length: 315 bp

>AT230032 NZ_CP124664:c144654-144427 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA