Detailed information of TA system TA230030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 521613..522217
• Replicon: plasmid pRLgr12_3
• Accession: NZ_CP090094
• Organism: Rhizobium leguminosarum strain Gr 12/7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LZK78_RS36875
• Protein ID: WP_128409492.1
• Coordinates: 521810..522217 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LZK78_RS36870
• Protein ID: WP_233944071.1
• Coordinates: 521613..521813 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK78_RS36850 (LZK78_36860)	516984..517130	-	147	WP_003552150.1	DUF1127 domain-containing protein	-
LZK78_RS36855 (LZK78_36865)	517583..518512	+	930	WP_130767242.1	TauD/TfdA family dioxygenase	-
LZK78_RS36860 (LZK78_36870)	518714..520090	-	1377	WP_128409493.1	FAD/NAD(P)-binding protein	-
LZK78_RS36865 (LZK78_36875)	520462..521310	+	849	WP_128409614.1	serine O-acetyltransferase	-
LZK78_RS36870 (LZK78_36880)	521613..521813	+	201	WP_233944071.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LZK78_RS36875 (LZK78_36885)	521810..522217	+	408	WP_128409492.1	type II toxin-antitoxin system VapC family toxin	Toxin
LZK78_RS36880 (LZK78_36890)	522532..522789	+	258	WP_128404570.1	CrpP-related protein	-
LZK78_RS36885 (LZK78_36895)	522884..524686	-	1803	WP_128409491.1	adenine deaminase C-terminal domain-containing protein	-
LZK78_RS36890 (LZK78_36900)	525088..526293	-	1206	WP_128409490.1	L-2-hydroxyglutarate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..643794	643794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14884.06 Da
• Isoelectric Point: 6.6046

>T230030 WP_128409492.1 NZ_CP090094:521810-522217 [Rhizobium leguminosarum]
VILADTSIWIDHFRHIDAELRRIIEDDRLLCHPSVIGELALGSLRDRGSVIAFLAAQRGAIVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASVLLDRRAALWTRDKRLRTAAEKAGASLHIPGSAPPLDAETDRQ

Nucleotide

• Length: 408 bp

>T230030 NZ_CP124663:c4545929-4545174 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7062.22 Da
• Isoelectric Point: 10.9131

>AT230030 WP_233944071.1 NZ_CP090094:521613-521813 [Rhizobium leguminosarum]
MRSTINLDDALLDKAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEVAPRRRSAAVK

Nucleotide

• Length: 201 bp

>AT230030 NZ_CP124663:c4546432-4546064 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTGGATGCGATCCGCGGCAAGGTCTGA