Detailed information of TA system TA230028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 187381..187913
• Replicon: plasmid pRLgr12_3
• Accession: NZ_CP090094
• Organism: Rhizobium leguminosarum strain Gr 12/7

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LZK78_RS35305
• Protein ID: WP_128400767.1
• Coordinates: 187381..187674 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: LZK78_RS35310
• Protein ID: WP_128400766.1
• Coordinates: 187671..187913 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK78_RS35290 (LZK78_35300)	183329..184342	+	1014	WP_128411067.1	alpha-ketoacid dehydrogenase subunit beta	-
LZK78_RS35295 (LZK78_35305)	184346..185593	+	1248	WP_128411066.1	dihydrolipoamide acetyltransferase family protein	-
LZK78_RS35300 (LZK78_35310)	185599..186996	+	1398	WP_128411065.1	dihydrolipoyl dehydrogenase	-
LZK78_RS35305 (LZK78_35315)	187381..187674	-	294	WP_128400767.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZK78_RS35310 (LZK78_35320)	187671..187913	-	243	WP_128400766.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LZK78_RS35315 (LZK78_35325)	188909..189307	+	399	WP_233944187.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..643794	643794

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11229.75 Da
• Isoelectric Point: 4.9134

>T230028 WP_128400767.1 NZ_CP090094:c187674-187381 [Rhizobium leguminosarum]
MSFKLSADAEEDIIAIAEQGVRMFGAGQAKRYHDELFALFDLIAVNPRIARERDEIDPPVRIHPFKAHLVVYRIEDDETI
FVIRIRHAHEDWATDSI

Nucleotide

• Length: 294 bp

>T230028 NZ_CP124663:1911614-1912201 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGACGTTGCCGCTCGCTGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGTGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8835.97 Da
• Isoelectric Point: 9.7765

>AT230028 WP_128400766.1 NZ_CP090094:c187913-187671 [Rhizobium leguminosarum]
MATMNVSLPGPMKDWVEAQARTGRYSNASDYVRDLIRRDQTRNDKIAAMQSFVEAGLQSGVGIRSKDELFAEAIARASKS

Nucleotide

• Length: 243 bp

>AT230028 NZ_CP124663:1912268-1913122 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGTGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGCTCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGAGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGAGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA