Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 942143..942933 Replicon plasmid pRL1
Accession NZ_CP090093
Organism Rhizobium leguminosarum strain Gr 12/7

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag LZK78_RS33265 Protein ID WP_128410208.1
Coordinates 942436..942933 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A444I704
Locus tag LZK78_RS33260 Protein ID WP_018494987.1
Coordinates 942143..942439 (+) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK78_RS33240 (LZK78_33250) 937151..937975 + 825 WP_130767536.1 ABC transporter permease -
LZK78_RS33245 (LZK78_33255) 937986..939617 + 1632 WP_222314855.1 ABC transporter ATP-binding protein -
LZK78_RS33250 (LZK78_33260) 939640..940860 + 1221 WP_233943375.1 M20/M25/M40 family metallo-hydrolase -
LZK78_RS33255 (LZK78_33265) 940863..941997 + 1135 Protein_886 Xaa-Pro peptidase family protein -
LZK78_RS33260 (LZK78_33270) 942143..942439 + 297 WP_018494987.1 DUF1778 domain-containing protein Antitoxin
LZK78_RS33265 (LZK78_33275) 942436..942933 + 498 WP_128410208.1 GNAT family N-acetyltransferase Toxin
LZK78_RS33270 (LZK78_33280) 943214..944785 - 1572 WP_128402217.1 amidohydrolase family protein -
LZK78_RS33275 (LZK78_33285) 944782..945831 - 1050 WP_128410206.1 questin oxidase family protein -
LZK78_RS33280 (LZK78_33290) 945873..946355 - 483 WP_128402215.1 nucleoside deaminase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1182792 1182792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(44-142)

Antitoxin

(16-90)


Sequences


Toxin        


Download         Length: 166 a.a.        Molecular weight: 17744.24 Da        Isoelectric Point: 6.9789

>T230027 WP_128410208.1 NZ_CP090093:942436-942933 [Rhizobium leguminosarum]
VTVSVPAPLADHHELAEFHSGVPELDDWLRLRARANQASGASRTFVVCEANRVIAYYALASGAVKQSEASGRFRRNMPDP
IPVAVLGRLAIDQSYQGRGFGRALVRDAGLRLINAAEILGIRGVLVHAISDDARAFYEAVGFLPSPSDPMMLLVGLHDLN
NALTS

Download         Length: 498 bp

>T230027 NZ_CP124663:1642433-1643302 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACACCCCCGTCCCGAACCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGTTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGTGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCTTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCTCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGTCGATTCCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11094.63 Da        Isoelectric Point: 7.0154

>AT230027 WP_018494987.1 NZ_CP090093:942143-942439 [Rhizobium leguminosarum]
MPTSHTSKSPDASVPLNMRIKPATRNLIDRAAEMLGKTRTDFMLEASERRAEEVLLDRTIFTVSAEVYAEYLARLDAPTQ
PNDRLKRTMSTKAPWDEA

Download         Length: 297 bp

>AT230027 NZ_CP124663:1643295-1644128 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTTTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTCGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A444I704

References