Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-StbC
Location 532684..533360 Replicon plasmid pRL1
Accession NZ_CP090093
Organism Rhizobium leguminosarum strain Gr 12/7

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LZK78_RS31265 Protein ID WP_128400695.1
Coordinates 532684..533109 (-) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag LZK78_RS31270 Protein ID WP_105009160.1
Coordinates 533106..533360 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZK78_RS31245 (LZK78_31255) 527940..528599 - 660 WP_128400699.1 sigma-70 family RNA polymerase sigma factor -
LZK78_RS31250 (LZK78_31260) 528762..529253 + 492 WP_128400698.1 GNAT family N-acetyltransferase -
LZK78_RS31255 (LZK78_31265) 529378..530166 - 789 WP_128409794.1 siderophore-iron reductase FhuF -
LZK78_RS31260 (LZK78_31270) 530170..532350 - 2181 WP_128400696.1 TonB-dependent siderophore receptor -
LZK78_RS31265 (LZK78_31275) 532684..533109 - 426 WP_128400695.1 type II toxin-antitoxin system VapC family toxin Toxin
LZK78_RS31270 (LZK78_31280) 533106..533360 - 255 WP_105009160.1 plasmid stabilization protein Antitoxin
LZK78_RS31275 (LZK78_31285) 533668..534240 - 573 WP_128400694.1 TetR/AcrR family transcriptional regulator -
LZK78_RS31280 (LZK78_31290) 534349..535035 + 687 WP_128400693.1 glutathione S-transferase -
LZK78_RS31285 (LZK78_31295) 535132..536103 + 972 WP_128400692.1 alpha/beta hydrolase -
LZK78_RS31290 (LZK78_31300) 536178..536582 - 405 WP_128400704.1 hypothetical protein -
LZK78_RS31295 (LZK78_31305) 536783..537208 + 426 WP_128400691.1 MarR family winged helix-turn-helix transcriptional regulator -
LZK78_RS31300 (LZK78_31310) 537276..538169 + 894 WP_128409795.1 NAD(P)H-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..1182792 1182792


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15272.64 Da        Isoelectric Point: 5.1351

>T230026 WP_128400695.1 NZ_CP090093:c533109-532684 [Rhizobium leguminosarum]
MILIDTNVISEPWKPTPDNQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARLAGRAIGKADAYIAAIAAANGFIVATRDTGPFEAAGLKVINPWYAPT

Download         Length: 426 bp

>T230026 NZ_CP124663:c143129-142848 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9262.47 Da        Isoelectric Point: 6.2235

>AT230026 WP_105009160.1 NZ_CP090093:c533360-533106 [Rhizobium leguminosarum]
MAAVTIRNLSEETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKTPAEP
MSFE

Download         Length: 255 bp

>AT230026 NZ_CP124663:c143353-143126 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References