Detailed information of TA system TA230024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1126160..1127646
• Replicon: chromosome
• Accession: NZ_CP090090
• Organism: Rhizobium leguminosarum strain Gr 12/7

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: -
• Locus tag: LZK78_RS05505
• Protein ID: WP_130766032.1
• Coordinates: 1126160..1127419 (-)
• Length: 420 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: A0A1B1C3T1
• Locus tag: LZK78_RS05510
• Protein ID: WP_065279083.1
• Coordinates: 1127416..1127646 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK78_RS05475 (LZK78_05475)	1121160..1122321	-	1162	Protein_1079	IS481 family transposase	-
LZK78_RS05480 (LZK78_05480)	1122438..1123355	-	918	WP_128403146.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LZK78_RS05485 (LZK78_05485)	1123345..1124193	-	849	WP_233942424.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
LZK78_RS05490 (LZK78_05490)	1124420..1124767	+	348	WP_233941996.1	DUF5655 domain-containing protein	-
LZK78_RS05495 (LZK78_05495)	1124760..1124864	+	105	Protein_1083	DUF429 domain-containing protein	-
LZK78_RS05500 (LZK78_05500)	1124955..1125734	-	780	WP_128403147.1	SDR family NAD(P)-dependent oxidoreductase	-
LZK78_RS05505 (LZK78_05505)	1126160..1127419	-	1260	WP_130766032.1	type II toxin-antitoxin system HipA family toxin	Toxin
LZK78_RS05510 (LZK78_05510)	1127416..1127646	-	231	WP_065279083.1	helix-turn-helix transcriptional regulator	Antitoxin
LZK78_RS05515 (LZK78_05515)	1128027..1128635	-	609	WP_128403149.1	TetR/AcrR family transcriptional regulator	-
LZK78_RS05520 (LZK78_05520)	1128747..1129433	+	687	WP_128403150.1	PspA/IM30 family protein	-
LZK78_RS05525 (LZK78_05525)	1129487..1129768	+	282	WP_003546080.1	YiaA/YiaB family inner membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 420 a.a.
• Molecular weight: 45389.22 Da
• Isoelectric Point: 6.2656

>T230024 WP_130766032.1 NZ_CP090090:c1127419-1126160 [Rhizobium leguminosarum]
MTSRLLNVWWDGRIVGQFTQDRHGDIGFVYARAWLDDDKTLPLSASLPKRPERFTRRECRPFFGGLLPEESQRLAAARAL
GVSPANDFALLDHLGGDVAGALQLLAEGEAPAGTGSIADRQPVPLDEAGIVAVLEALPVRPLLAGEEGLRLSLAGAQSKL
PVVLVDGRIGLPLPGQATTHILKPPIARFKATTENEAFVMRLAAAIALDVAAVEPRYVQGRPFLLVERYDRLRGAGGLVH
RIHQEDFCQALSVPPETKYASEGGPTFNCFDLIRRISARPAVDVLKLLDAAIFNLIAGNADAHGKNFSILYDSQGPRLAK
LYDLLSTVIYPELSPKLAMRLGKRATLAEMDAEGWKVFAGEAGVGYPLFRRRVNELCDKVLTLTGKVADELADVSPDRDP
IDAIAGVITDRAETARLTL

Nucleotide

• Length: 1260 bp

>T230024 NZ_CP124662:c5843993-5843694 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8133.43 Da
• Isoelectric Point: 9.3464

>AT230024 WP_065279083.1 NZ_CP090090:c1127646-1127416 [Rhizobium leguminosarum]
MAKHVQSPADIGALVRSTRKEQNLRQDELAGVAGVGLRFIVDLEAGKPTAQIGKVLQVLQTLGCSLDILAPGERRQ

Nucleotide

• Length: 231 bp

>AT230024 NZ_CP124662:c5843697-5843407 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1B1C3T1