Detailed information of TA system TA230022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2100500..2101478
• Replicon: chromosome
• Accession: NZ_CP090081
• Organism: Bacillus cereus strain CPT56D-587-MTF

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: LW858_RS10760
• Protein ID: WP_000624977.1
• Coordinates: 2100500..2101237 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: LW858_RS10765
• Protein ID: WP_000237819.1
• Coordinates: 2101350..2101478 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW858_RS10740 (LW858_10740)	2096142..2096924	+	783	WP_233936358.1	class I SAM-dependent methyltransferase	-
LW858_RS10745 (LW858_10745)	2097082..2098767	-	1686	WP_065212732.1	alpha-keto acid decarboxylase family protein	-
LW858_RS10750 (LW858_10750)	2098875..2099357	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
LW858_RS10755 (LW858_10755)	2099524..2100261	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
LW858_RS10760 (LW858_10760)	2100500..2101237	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LW858_RS10765 (LW858_10765)	2101350..2101478	+	129	WP_000237819.1	hypothetical protein	Antitoxin
LW858_RS10770 (LW858_10770)	2101551..2101727	+	177	WP_233936359.1	stage II sporulation protein SB	-
LW858_RS10775 (LW858_10775)	2101748..2102137	-	390	WP_065212731.1	YxeA family protein	-
LW858_RS10780 (LW858_10780)	2102348..2103817	+	1470	WP_065212730.1	beta-Ala-His dipeptidase	-
LW858_RS10785 (LW858_10785)	2104062..2104871	+	810	WP_233936360.1	papain-like cysteine protease family protein	-
LW858_RS10790 (LW858_10790)	2104897..2105499	+	603	WP_233936361.1	hypothetical protein	-
LW858_RS10795 (LW858_10795)	2105743..2105994	-	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T230022 WP_000624977.1 NZ_CP090081:2100500-2101237 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T230022 NZ_CP124662:c5055390-5055091 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT230022 WP_000237819.1 NZ_CP090081:2101350-2101478 [Bacillus cereus]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT230022 NZ_CP124662:c5055094-5054804 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2