Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4531591..4532210 Replicon chromosome
Accession NZ_CP090078
Organism Klebsiella michiganensis strain KM41

Toxin (Protein)


Gene name Hha Uniprot ID J5X5R4
Locus tag LW125_RS21805 Protein ID WP_004848333.1
Coordinates 4531992..4532210 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J4WQ74
Locus tag LW125_RS21800 Protein ID WP_009653563.1
Coordinates 4531591..4531965 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LW125_RS21790 4526750..4527943 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LW125_RS21795 4527966..4531112 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit AcrB -
LW125_RS21800 4531591..4531965 + 375 WP_009653563.1 Hha toxicity modulator TomB Antitoxin
LW125_RS21805 4531992..4532210 + 219 WP_004848333.1 HHA domain-containing protein Toxin
LW125_RS21810 4532372..4532938 + 567 WP_004848331.1 maltose O-acetyltransferase -
LW125_RS21815 4532910..4533044 - 135 WP_223226670.1 hypothetical protein -
LW125_RS21820 4533065..4533535 + 471 WP_004848329.1 YlaC family protein -
LW125_RS21825 4533510..4534964 - 1455 WP_086530504.1 PLP-dependent aminotransferase family protein -
LW125_RS21830 4535066..4535764 + 699 WP_128349260.1 GNAT family protein -
LW125_RS21835 4535761..4535901 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
LW125_RS21840 4535901..4536164 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8626.03 Da        Isoelectric Point: 8.9008

>T230018 WP_004848333.1 NZ_CP090078:4531992-4532210 [Klebsiella michiganensis]
MSDKTLTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T230018 NZ_CP124662:2620357-2620650 [Pseudomonas aeruginosa]
GTGCCACGACTGATTGTCACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCCGATAAAGATCCGCA
AGTCGCGCGCCGCGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGCCCATTTC
CAGATCTGCCTGAGCTGCGTGAGCTCATCATCGAGTTTGGGGACTCAGGTTACGTCGCGCTGTATCGCTATGAGCGCGCA
GATGACACAGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14378.14 Da        Isoelectric Point: 4.8989

>AT230018 WP_009653563.1 NZ_CP090078:4531591-4531965 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT230018 NZ_CP124662:2620097-2620369 [Pseudomonas aeruginosa]
ATGGCCACTTCCATCAAAATTGACGACGAGCTGAAAGGTCGCATTCAGCATTTGGCTGGTTTGCGGCAGCGTTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGCGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCGTATCAGGAAACCGGCAGGCATTTGACCGGCCAAGAGGCTCGCACCTGGCTTGGTAGCTGGGGTACAGAT
GCTGAGGCAGAGCTTCCCAAGTGCCACGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R4L0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R4I8

References