Detailed information of TA system TA230009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2024199..2024789
• Replicon: chromosome
• Accession: NZ_CP090078
• Organism: Klebsiella michiganensis strain KM41

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LW125_RS09615
• Protein ID: WP_128350044.1
• Coordinates: 2024457..2024789 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: J6I3K7
• Locus tag: LW125_RS09610
• Protein ID: WP_004852307.1
• Coordinates: 2024199..2024456 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW125_RS09590	2020097..2020702	-	606	WP_004852315.1	glutathione S-transferase family protein	-
LW125_RS09595	2020882..2021808	+	927	WP_049078228.1	LysR substrate-binding domain-containing protein	-
LW125_RS09600	2021846..2022844	-	999	WP_009653340.1	aldo/keto reductase	-
LW125_RS09605	2022945..2023859	+	915	WP_086531142.1	LysR family transcriptional regulator	-
LW125_RS09610	2024199..2024456	+	258	WP_004852307.1	antitoxin	Antitoxin
LW125_RS09615	2024457..2024789	+	333	WP_128350044.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LW125_RS09625	2025112..2026569	+	1458	WP_032743138.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
LW125_RS09635	2027532..2028986	-	1455	WP_004852297.1	AMP nucleosidase	-
LW125_RS09640	2029118..2029375	-	258	WP_004852295.1	histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2015134..2024789	9655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11837.72 Da
• Isoelectric Point: 10.5834

>T230009 WP_128350044.1 NZ_CP090078:2024457-2024789 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSAGNFARTAGFTVSLEGAGIKTTGVIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Nucleotide

• Length: 333 bp

>T230009 NZ_CP124660:c144265-143984 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9422.88 Da
• Isoelectric Point: 4.4607

>AT230009 WP_004852307.1 NZ_CP090078:2024199-2024456 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT230009 NZ_CP124660:c144489-144262 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3HDW8