Detailed information of TA system TA230008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1362886..1363520
• Replicon: chromosome
• Accession: NZ_CP090078
• Organism: Klebsiella michiganensis strain KM41

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LW125_RS06595
• Protein ID: WP_006687278.1
• Coordinates: 1363116..1363520 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LW125_RS06590
• Protein ID: WP_220151913.1
• Coordinates: 1362886..1363116 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW125_RS06580	1359054..1360583	+	1530	WP_220151915.1	AAA family ATPase	-
LW125_RS06585	1361031..1361906	-	876	WP_220151914.1	integrase domain-containing protein	-
LW125_RS06590	1362886..1363116	+	231	WP_220151913.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LW125_RS06595	1363116..1363520	+	405	WP_006687278.1	tRNA(fMet)-specific endonuclease VapC	Toxin
LW125_RS06600	1363543..1364766	-	1224	WP_220151912.1	radical SAM protein	-
LW125_RS06605	1365156..1366397	+	1242	WP_220151965.1	MFS transporter	-
LW125_RS06610	1366457..1367470	-	1014	WP_220151964.1	Gfo/Idh/MocA family oxidoreductase	-
LW125_RS06615	1367481..1368461	-	981	WP_220151911.1	PTS glucitol/sorbitol transporter subunit IIB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1348437..1379616	31179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14977.31 Da
• Isoelectric Point: 7.8441

>T230008 WP_006687278.1 NZ_CP090078:1363116-1363520 [Klebsiella michiganensis]
MLKYMLDTNICIYTIKNKPQAVREAFNQHYGRMCISSVTLMELIYGAEKSASPEKNLRVVEGFIARLEVLNYGIDAAVQT
GQIRAELARAGTPVGPYDSMIAAHARALGLILVTNNTREFERINGLRLEDWSIS

Nucleotide

• Length: 405 bp

>T230008 NZ_CP124659:244460-244984 [Pseudomonas aeruginosa]
ATGAAAATCATCGGAGTGCAGGACCTCCCCTGCTACCGAATGCACACTCCCAAGTGGTCCGTTGCGCCTACATCTGGAGC
TGGAGCTGCAACTCAGGGTGGCCGGGTTAACCGAAAGGGGCTAGAGGCTCTATACCTCTCGCTGGAGTCGGCAACGGCCA
TTGCTGAGTTTCAGCAAGCAAGCGCATTCCTTCCACCAGGTCTGCTGGTCAGCTATTCCCTCAGTCTGGATCGAGTTATC
GACTTCACCGGCGGCTATGACGATACCTGGGCCCCGATCTGGCAAGACTTCTTCTGCGACTGGAGAAAGATTCGCTTCAA
CGACGGCGTTGAACCGCCGAGCTGGATTATCGGGGATCTGGCTATCGAGGCTGACTGTGCTGGGATTCTGTTCGAGTCCG
TTGCGAACCCTGGAGGTCGGAACCTGGTGCTTTTCACCGATCAGTTGCCGGTGCATGGGAATATTGTGGTCAACGATCCT
CGCGGGGATCTCCCTACCGACCAGTCATCTTGGATGCGCCCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8479.46 Da
• Isoelectric Point: 4.7672

>AT230008 WP_220151913.1 NZ_CP090078:1362886-1363116 [Klebsiella michiganensis]
MEKTTVFKSNRSQAVRLPKAVALPEDVKHVDIVAVGRTRIITPAGESWDSWFDGESVTSDFMAAREQPGEQDREGF

Nucleotide

• Length: 231 bp

>AT230008 NZ_CP124659:244068-244463 [Pseudomonas aeruginosa]
ATGGCCAGCAAAGCCGTATTAGAGCTTCGCCCCACCAACTTCGCGGGCTTGATGGACGCTCTCAAAGAGGTGAACCGTGT
GATGTTCTCCCCTGAGCGCTTCCTCAAGGTTTTTTCGATGGACCAGCAGACCTTGGCGCTCCGGGCCCATGTCCATCGCA
ACACTGTGCGCAACGCGCCTGAATCGGAAAAGGTCCAGGCCTACATTCGTGATTCAGTGAAGGTTCTGCGTGCAGTGACC
GACATGGGCACGGACGTGACGAATGCCATCTTCTGGTTTAAGAACGAGCCTCTTTCCACGTTCAACTATAAGACCGCGGA
GGAAGTGGTCAGCGAAGGAAAAACCGAGCAGCTGATCGCTTTCCTTCAATCCTGGGAAGCTGGTGCCCAAGGATGA