Detailed information of TA system TA230005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 48971..49499
• Replicon: plasmid pYUSHP14-1
• Accession: NZ_CP090068
• Organism: Acinetobacter sp. SH20PTE14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A4R0ERS7
• Locus tag: LXF01_RS16745
• Protein ID: WP_038349496.1
• Coordinates: 48971..49264 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: LXF01_RS16750
• Protein ID: WP_000246756.1
• Coordinates: 49254..49499 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXF01_RS16720 (LXF01_16720)	45092..45892	+	801	WP_151204773.1	hypothetical protein	-
LXF01_RS16725 (LXF01_16725)	46887..47201	-	315	WP_180102757.1	BrnA antitoxin family protein	-
LXF01_RS16730 (LXF01_16730)	47188..47475	-	288	WP_039890878.1	BrnT family toxin	-
LXF01_RS16735 (LXF01_16735)	47734..48264	-	531	WP_148335254.1	HPP family protein	-
LXF01_RS16740 (LXF01_16740)	48361..48900	+	540	WP_233945562.1	TetR/AcrR family transcriptional regulator	-
LXF01_RS16745 (LXF01_16745)	48971..49264	-	294	WP_038349496.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXF01_RS16750 (LXF01_16750)	49254..49499	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LXF01_RS16755 (LXF01_16755)	49661..50350	+	690	WP_219191771.1	aspartate/glutamate racemase family protein	-
LXF01_RS16760 (LXF01_16760)	50762..51022	+	261	WP_180036186.1	helix-turn-helix domain-containing protein	-
LXF01_RS16765 (LXF01_16765)	51341..51643	+	303	WP_111886029.1	helix-turn-helix transcriptional regulator	-
LXF01_RS16770 (LXF01_16770)	51648..52907	+	1260	WP_233945563.1	type II toxin-antitoxin system HipA family toxin	-
LXF01_RS16775 (LXF01_16775)	53174..53356	-	183	Protein_55	transposase	-
LXF01_RS16780 (LXF01_16780)	53410..54342	-	933	Protein_56	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(X3) / floR / aph(6)-Id / aph(3'')-Ib	-	1..187536	187536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11320.54 Da
• Isoelectric Point: 10.5074

>T230005 WP_038349496.1 NZ_CP090068:c49264-48971 [Acinetobacter sp. SH20PTE14]
MTYKLLRHKDFTAEWERLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T230005 NZ_CP124658:c4841713-4841414 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT230005 WP_000246756.1 NZ_CP090068:c49499-49254 [Acinetobacter sp. SH20PTE14]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT230005 NZ_CP124658:c4841417-4841127 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A4R0ERS7

Antitoxin

• AlphaFold DB: N9B5Q7