Detailed information of TA system TA230004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 46887..47475
• Replicon: plasmid pYUSHP14-1
• Accession: NZ_CP090068
• Organism: Acinetobacter sp. SH20PTE14

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: LXF01_RS16730
• Protein ID: WP_039890878.1
• Coordinates: 47188..47475 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: LXF01_RS16725
• Protein ID: WP_180102757.1
• Coordinates: 46887..47201 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXF01_RS16700 (LXF01_16700)	42428..42982	+	555	WP_086044968.1	hypothetical protein	-
LXF01_RS16705 (LXF01_16705)	43132..43359	-	228	WP_180024629.1	hypothetical protein	-
LXF01_RS16710 (LXF01_16710)	43395..43751	-	357	WP_180025034.1	hypothetical protein	-
LXF01_RS16715 (LXF01_16715)	43908..44840	+	933	Protein_43	IS5 family transposase	-
LXF01_RS16720 (LXF01_16720)	45092..45892	+	801	WP_151204773.1	hypothetical protein	-
LXF01_RS16725 (LXF01_16725)	46887..47201	-	315	WP_180102757.1	BrnA antitoxin family protein	Antitoxin
LXF01_RS16730 (LXF01_16730)	47188..47475	-	288	WP_039890878.1	BrnT family toxin	Toxin
LXF01_RS16735 (LXF01_16735)	47734..48264	-	531	WP_148335254.1	HPP family protein	-
LXF01_RS16740 (LXF01_16740)	48361..48900	+	540	WP_233945562.1	TetR/AcrR family transcriptional regulator	-
LXF01_RS16745 (LXF01_16745)	48971..49264	-	294	WP_038349496.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LXF01_RS16750 (LXF01_16750)	49254..49499	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
LXF01_RS16755 (LXF01_16755)	49661..50350	+	690	WP_219191771.1	aspartate/glutamate racemase family protein	-
LXF01_RS16760 (LXF01_16760)	50762..51022	+	261	WP_180036186.1	helix-turn-helix domain-containing protein	-
LXF01_RS16765 (LXF01_16765)	51341..51643	+	303	WP_111886029.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / tet(X3) / floR / aph(6)-Id / aph(3'')-Ib	-	1..187536	187536

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11263.65 Da
• Isoelectric Point: 5.2216

>T230004 WP_039890878.1 NZ_CP090068:c47475-47188 [Acinetobacter sp. SH20PTE14]
MEQYFEWDEAKNRKNQKKHDVSFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Nucleotide

• Length: 288 bp

>T230004 NZ_CP124658:c4841417-4841127 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12255.91 Da
• Isoelectric Point: 7.2029

>AT230004 WP_180102757.1 NZ_CP090068:c47201-46887 [Acinetobacter sp. SH20PTE14]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGIVPDDQLDLSDIPEMTDWSNAARHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT230004 NZ_CP124658:c4841713-4841414 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA