Detailed information of TA system TA229997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3328203..3329437
• Replicon: chromosome
• Accession: NZ_CP090065
• Organism: Pectobacterium polonicum strain BY21311

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LW347_RS15025
• Protein ID: WP_258882757.1
• Coordinates: 3328203..3329123 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LW347_RS15030
• Protein ID: WP_137740328.1
• Coordinates: 3329120..3329437 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW347_RS15010 (LW347_14990)	3323893..3325965	+	2073	WP_258882755.1	polyphosphate kinase 1	-
LW347_RS15015 (LW347_14995)	3325965..3327500	+	1536	WP_258882756.1	exopolyphosphatase	-
LW347_RS15020 (LW347_15000)	3327732..3327920	+	189	WP_137740326.1	YjfB family protein	-
LW347_RS15025 (LW347_15005)	3328203..3329123	+	921	WP_258882757.1	HipA domain-containing protein	Toxin
LW347_RS15030 (LW347_15010)	3329120..3329437	+	318	WP_137740328.1	helix-turn-helix domain-containing protein	Antitoxin
LW347_RS15035 (LW347_15015)	3329578..3329793	+	216	WP_107170869.1	YfgG family protein	-
LW347_RS15040 (LW347_15020)	3329979..3330404	-	426	WP_137740329.1	organic hydroperoxide resistance protein	-
LW347_RS15045 (LW347_15025)	3330564..3331013	-	450	WP_137740330.1	MarR family transcriptional regulator	-
LW347_RS15050 (LW347_15030)	3331233..3332711	+	1479	WP_258882758.1	magnesium transporter	-
LW347_RS15055 (LW347_15035)	3332827..3333009	+	183	Protein_2946	type II toxin-antitoxin system HicA family toxin	-
LW347_RS15060 (LW347_15040)	3333174..3333611	+	438	WP_039471078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (28-250)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-86)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34706.51 Da
• Isoelectric Point: 4.9644

>T229997 WP_258882757.1 NZ_CP090065:3328203-3329123 [Pectobacterium polonicum]
VQDVADRNSDFIEYAQQRGAAGGGATGAGGEAPKLLLRCSDNDEIWIDPFQDDLSTQDMHYLVKFPRGPRSPVDCDILRA
EYYFYHELTLLGMETINLEGMRLIEGEHYPSLWLPRFDVDYIDGRKTLHGMESIYSLLKKPAGSYLNHFTVIQRIIDTIA
PPTGNHAPQRRELVIEMVKRDMLNIIFGNSDNHGRNTSLIKRPQGIWLAPIYDFAPMKADPEGIARTTQWGSPYEEGGRI
DWFAIANALSSFIDPEDLIHELSTTARQLIGLKSRLIERGVPQRIIAMPVMAFDYIDEKLATWQLL

Nucleotide

• Length: 921 bp

>T229997 NZ_CP124657:c5815050-5814772 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12010.92 Da
• Isoelectric Point: 10.3089

>AT229997 WP_137740328.1 NZ_CP090065:3329120-3329437 [Pectobacterium polonicum]
MKNLSSSERDALLAHLLTEFFNERLSIGGLLHKLRKDVFGMSQEQYATLVEVSRRTLSDIEKDRGSQSIMLLNRVFRPLG
LKVGLLPRSTHLMYDLLEKGNHSKN

Nucleotide

• Length: 318 bp

>AT229997 NZ_CP124657:c5814760-5814455 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG