Detailed information of TA system TA229993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1328748..1329373
• Replicon: chromosome
• Accession: NZ_CP090065
• Organism: Pectobacterium polonicum strain BY21311

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: C6DB72
• Locus tag: LW347_RS05975
• Protein ID: WP_005976087.1
• Coordinates: 1328748..1328951 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: LW347_RS05980
• Protein ID: WP_258884490.1
• Coordinates: 1329005..1329373 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW347_RS05945 (LW347_05930)	1324071..1324409	+	339	WP_002208627.1	P-II family nitrogen regulator	-
LW347_RS05950 (LW347_05935)	1324449..1325735	+	1287	WP_165800659.1	ammonium transporter AmtB	-
LW347_RS05955 (LW347_05940)	1325819..1326682	-	864	WP_137739376.1	acyl-CoA thioesterase II	-
LW347_RS05960 (LW347_05945)	1326895..1327479	+	585	WP_137739377.1	YbaY family lipoprotein	-
LW347_RS05965 (LW347_05950)	1327499..1327819	-	321	WP_137739378.1	MGMT family protein	-
LW347_RS05975 (LW347_05960)	1328748..1328951	-	204	WP_005976087.1	HHA domain-containing protein	Toxin
LW347_RS05980 (LW347_05965)	1329005..1329373	-	369	WP_258884490.1	Hha toxicity modulator TomB	Antitoxin
LW347_RS05985 (LW347_05970)	1329970..1330113	-	144	WP_005976091.1	type B 50S ribosomal protein L36	-
LW347_RS05990 (LW347_05975)	1330131..1330379	-	249	WP_137739379.1	type B 50S ribosomal protein L31	-
LW347_RS05995 (LW347_05980)	1330608..1333736	-	3129	WP_137739380.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8145.52 Da
• Isoelectric Point: 8.9008

>T229993 WP_005976087.1 NZ_CP090065:c1328951-1328748 [Pectobacterium polonicum]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFFSAADHRLAELTMNKLYDKVPTAVWRYVR

Nucleotide

• Length: 204 bp

>T229993 NZ_CP124657:1647506-1648375 [Pseudomonas aeruginosa]
ATGAACATGGCGCAGACCCACATCCCCGCCCCGAGCCTGCAACAGCGCTTTCCCGAGCGGCTGGTGCAACTGGCCGACGG
CGCCCAGCTGGCCATCCGCGAATGCGGCCAGGGCCCGGTGGTGGTGCTGTTGCACGGCATCGGTTCGGGCTCGGCGTCCT
GGCTGCATTGCGCCCAGCGCCTGGCCGCCGGCAATCGGGTGATCGCCTGGGACGCGCCGGGCTACGGCCTGTCCACGCCG
CTGCCGCCGGCCCGGCCCAAGGCCTGCGACTACGCCGCGCGCCTGGAGCTGCTGCTGGATGCGCTGGGCGTGGAGAGCTG
CCTGCTGGTGGGCCACTCCCTCGGCGCGCTGATGGCCACGGCCTACGCCGCCGGCATCGGCGCGGCGCGGGTGCGCCGCC
TGGTGCTGCTCAGCCCGGCGCGCGGCTACGGCGCCGCCGAGCTGCGCGACAGCGGCGCCCAGGTGCGCCGCCAGCGCCTG
GAGAACCTGGAGCGCCTCGGTATCGACGGCATGGCCAGCGAGCGCACCGCGCGCCTGCTCGGCCGCAACCCCAGCGAGGA
GGCCCTGGCCTGGGTGCGCTGGAACATGGCGCGGCTCAACCCCGAGGGCTACCGCCAGGCCGTCGAGCTGCTCTGCGGCG
ACGACCTGCTGGGCAACGGGCAGCCTGCCGCCCCTTGCGAGGTGCATTGCGGCGAGGACGACGGCATCACCACGCCCGAG
AGTTGCGGCGCCATCGCCCGCCAGCTCGGCGCGTCGTTCTCGCCGATTTCCGGGGCCGGCCACGCCAGCCCGATCGAACA
ACCCGAAGTGGTCGCAGGACGCATCGGCCACGCGCAACGCCTCTCTCTTGAAGGAACCGCCAATGGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14009.73 Da
• Isoelectric Point: 4.3697

>AT229993 WP_258884490.1 NZ_CP090065:c1329373-1329005 [Pectobacterium polonicum]
MDEYTPKNYDIAQLRFLCENLCDESIATLGDSSHGWVNDPTSAINLQLNELIEHIATFILTFKIKYPNESELSEQVEKYL
DDTYVLFSNYGINDAELRRWQKSKAKLFGMFSGENVCTPAKT

Nucleotide

• Length: 369 bp

>AT229993 NZ_CP124657:1648368-1649201 [Pseudomonas aeruginosa]
ATGGATAAGTCAGACGATTCGCAAGACAAGTACATCGTGCCTGGCCTCGAACGCGGCCTGCTGCTGCTCTGCGAGTTCAG
CCGGAAGGACCGTACCCTGACGGCGCCGGAGTTGGCGCGGCGCCTGAAGCTGCCGCGCTCGACCATCTTCCGCCTGCTGA
CCACCCTGGAGGCCATGGGCTTCGTCACCCGCAACGGCAACGAGTACCGCCTGGGCATGGCGGTGTTGCGCCTGGGCTTC
GAGTACCTGGCCTCGCTGGAGCTGACCGAACTCGGCCAGCCGCTGCTGAACCGCCTGTGCGACGAGATCCGCTACCCCTG
CAACCTGGTGGTGCGCGACGGCCGTTCGATCGTCTACGTGGCCAAGGTCTCGCCGTCCACCCCGCTGAGCAGCTCGGTGA
ACGTCGGGACCCGGTTGCCGGCCCACGCCACGGTGCTCGGCCGCATCCTGCTGCAGGACCTGAGCCTGGGCGAGCTGCGC
GAGCTGTACCCGGAGGAGCAGCTGGAGCAGTTCTCGCCGAACACCCCGCGCAGCGTGCTGGAACTCTTCGACATGGTCCA
GGGCGACCGCCAGCGTGGCTTCGTCCAGGGCGAGGGGTTCTTCGAGGCGAGCATCTCCACCGTTGCGGCGCCGGTACGTG
ATCACAGCGGTCGCGTCATCGCGGCGATGGGGGCGACCATCGCCGCCGGGCATATCGACCCGGAACGCATCGAGGGACTG
GTCAGCCGGGTGCGCAGCAGCGCCGACGAGCTGTCGTACCTGCTCGACTACCGCGCCGACGGCCAGGACAACGTCACCCC
GATCTTCCGGAGTCGCCCGCATGAAACCGTATGA

Structures

Toxin

• AlphaFold DB: A0A1D7Z3F0