Detailed information of TA system TA229992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1015503..1016287
• Replicon: chromosome
• Accession: NZ_CP090065
• Organism: Pectobacterium polonicum strain BY21311

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: LW347_RS04550
• Protein ID: WP_258884339.1
• Coordinates: 1015793..1016287 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: LW347_RS04545
• Protein ID: WP_100683471.1
• Coordinates: 1015503..1015796 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW347_RS04510 (LW347_04505)	1010781..1011332	-	552	WP_137741117.1	copper-binding periplasmic metallochaperone CueP	-
LW347_RS04515 (LW347_04510)	1011552..1011953	-	402	WP_258884336.1	VOC family protein	-
LW347_RS04520 (LW347_04515)	1012039..1012611	-	573	WP_258884337.1	TetR/AcrR family transcriptional regulator	-
LW347_RS04525 (LW347_04520)	1012742..1013275	+	534	WP_258884338.1	HPP family protein	-
LW347_RS04530 (LW347_04525)	1013338..1013472	+	135	Protein_873	flavodoxin family protein	-
LW347_RS04535 (LW347_04530)	1014501..1015007	-	507	WP_137741121.1	GNAT family N-acetyltransferase	-
LW347_RS04540 (LW347_04535)	1015141..1015233	+	93	Protein_875	transcriptional regulator	-
LW347_RS04545 (LW347_04540)	1015503..1015796	+	294	WP_100683471.1	DUF1778 domain-containing protein	Antitoxin
LW347_RS04550 (LW347_04545)	1015793..1016287	+	495	WP_258884339.1	GNAT family N-acetyltransferase	Toxin
LW347_RS04555 (LW347_04550)	1016351..1016509	-	159	Protein_878	Arm DNA-binding domain-containing protein	-
LW347_RS04560 (LW347_04555)	1016798..1017685	+	888	WP_258884340.1	ParA family protein	-
LW347_RS04565 (LW347_04560)	1017682..1018071	+	390	WP_258884341.1	hypothetical protein	-
LW347_RS04570 (LW347_04565)	1018064..1019424	+	1361	Protein_881	SPI-7-type island replicative DNA helicase	-
LW347_RS04575 (LW347_04570)	1019424..1021154	+	1731	WP_258884342.1	ParB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17610.56 Da
• Isoelectric Point: 8.7451

>T229992 WP_258884339.1 NZ_CP090065:1015793-1016287 [Pectobacterium polonicum]
MISAPEPLRAKHVLSSFCCGVESMDNWLKLRAMKNQVTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIHVADTIGIRGMLVHALSDEARAFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T229992 NZ_CP124657:c143250-142969 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10900.50 Da
• Isoelectric Point: 10.1968

>AT229992 WP_100683471.1 NZ_CP090065:1015503-1015796 [Pectobacterium polonicum]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPSPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT229992 NZ_CP124657:c143474-143247 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA