Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 6139568..6140136 Replicon chromosome
Accession NZ_CP090063
Organism Amycolatopsis acidiphila strain KCTC 39523

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LWP59_RS30080 Protein ID WP_186383315.1
Coordinates 6139568..6139918 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag LWP59_RS30085 Protein ID WP_144640498.1
Coordinates 6139915..6140136 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWP59_RS30065 (LWP59_30065) 6136179..6136775 + 597 WP_144640486.1 TetR/AcrR family transcriptional regulator -
LWP59_RS30070 (LWP59_30070) 6137133..6137600 - 468 WP_144640489.1 hypothetical protein -
LWP59_RS30075 (LWP59_30075) 6137597..6139087 - 1491 WP_144640492.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
LWP59_RS30080 (LWP59_30080) 6139568..6139918 - 351 WP_186383315.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LWP59_RS30085 (LWP59_30085) 6139915..6140136 - 222 WP_144640498.1 ribbon-helix-helix protein, CopG family Antitoxin
LWP59_RS30090 (LWP59_30090) 6140351..6141079 + 729 WP_186383316.1 amino acid ABC transporter ATP-binding protein -
LWP59_RS30095 (LWP59_30095) 6141135..6142043 + 909 WP_144640505.1 glutamate ABC transporter substrate-binding protein -
LWP59_RS30100 (LWP59_30100) 6142136..6142783 + 648 WP_144640507.1 amino acid ABC transporter permease -
LWP59_RS30105 (LWP59_30105) 6142780..6143664 + 885 WP_144640510.1 amino acid ABC transporter permease -
LWP59_RS30110 (LWP59_30110) 6143668..6145044 - 1377 WP_144640512.1 glycogen debranching protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12474.51 Da        Isoelectric Point: 11.1686

>T229982 WP_186383315.1 NZ_CP090063:c6139918-6139568 [Amycolatopsis acidiphila]
MMRRAEIRLVDLEPVRGAESSKTRPAVIVSNDGANGAASRLGRGVVTVVPVTTNVTRVYPFQVLLRAEECGLKVDSKAQA
EQVRSVAVERIGRKTGQVPASVMAKLDQALRVHLGL

Download         Length: 351 bp

>T229982 NZ_CP124654:c5417577-5417299 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8110.92 Da        Isoelectric Point: 4.0202

>AT229982 WP_144640498.1 NZ_CP090063:c6140136-6139915 [Amycolatopsis acidiphila]
MKLSVSLGEEDVAFLDEYAARTQAGSRSAVLHRAIELLRASQLENAYQAAWEEWEVEEGELWDATTGDGLAVR

Download         Length: 222 bp

>AT229982 NZ_CP124654:c5417287-5416982 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References