Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 50980..51716 Replicon plasmid pKPN-NE368
Accession NZ_CP090062
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-NE368

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LRM38_RS27465 Protein ID WP_003026803.1
Coordinates 51234..51716 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LRM38_RS27460 Protein ID WP_003026799.1
Coordinates 50980..51246 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM38_RS27415 (LRM38_27390) 47042..47404 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
LRM38_RS27420 (LRM38_27395) 47454..47804 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
LRM38_RS27425 (LRM38_27400) 48162..48431 + 270 WP_004152102.1 hypothetical protein -
LRM38_RS27430 (LRM38_27405) 48419..48994 + 576 WP_004152103.1 hypothetical protein -
LRM38_RS27435 (LRM38_27410) 49025..49519 + 495 WP_004152104.1 DNA-binding protein -
LRM38_RS27440 (LRM38_27415) 49563..49931 + 369 WP_004152105.1 hypothetical protein -
LRM38_RS27445 (LRM38_27420) 49965..50168 + 204 WP_004152106.1 HHA domain-containing protein -
LRM38_RS27450 (LRM38_27425) 50217..50474 + 258 WP_004152107.1 hypothetical protein -
LRM38_RS27455 (LRM38_27430) 50550..50804 + 255 WP_004152108.1 hypothetical protein -
LRM38_RS27460 (LRM38_27435) 50980..51246 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LRM38_RS27465 (LRM38_27440) 51234..51716 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LRM38_RS27470 (LRM38_27445) 51928..53274 + 1347 WP_020314316.1 ISNCY family transposase -
LRM38_RS27475 53435..53566 + 132 WP_004218042.1 hypothetical protein -
LRM38_RS27480 (LRM38_27450) 53775..54924 - 1150 Protein_56 IS3 family transposase -
LRM38_RS27485 (LRM38_27455) 55116..56078 - 963 WP_004152113.1 zinc metalloprotease HtpX -
LRM38_RS27490 (LRM38_27460) 56065..56553 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib / mph(A) / sul1 / qacE / aadA2 / dfrA12 / blaKPC-34 - 1..189603 189603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T229981 WP_003026803.1 NZ_CP090062:51234-51716 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T229981 NZ_CP124654:c4545135-4544380 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGCCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT229981 WP_003026799.1 NZ_CP090062:50980-51246 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT229981 NZ_CP124654:c4545638-4545270 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTGCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References