Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3257244..3257869 Replicon chromosome
Accession NZ_CP090061
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-NE368

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LRM38_RS16260 Protein ID WP_002882817.1
Coordinates 3257244..3257627 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LRM38_RS16265 Protein ID WP_004150355.1
Coordinates 3257627..3257869 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM38_RS16245 (LRM38_16230) 3254610..3255512 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LRM38_RS16250 (LRM38_16235) 3255509..3256144 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LRM38_RS16255 (LRM38_16240) 3256141..3257070 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LRM38_RS16260 (LRM38_16245) 3257244..3257627 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LRM38_RS16265 (LRM38_16250) 3257627..3257869 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LRM38_RS16270 (LRM38_16255) 3258074..3258991 + 918 WP_002882812.1 alpha/beta hydrolase -
LRM38_RS16275 (LRM38_16260) 3259005..3259946 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LRM38_RS16280 (LRM38_16265) 3259991..3260428 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LRM38_RS16285 (LRM38_16270) 3260425..3261285 - 861 WP_002882807.1 virulence factor BrkB family protein -
LRM38_RS16290 (LRM38_16275) 3261279..3261878 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229968 WP_002882817.1 NZ_CP090061:c3257627-3257244 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229968 NZ_CP124651:c6018607-6018329 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229968 WP_004150355.1 NZ_CP090061:c3257869-3257627 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229968 NZ_CP124651:c6018317-6018012 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References