Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2059403..2060022 Replicon chromosome
Accession NZ_CP090061
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-NE368

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LRM38_RS10510 Protein ID WP_002892050.1
Coordinates 2059804..2060022 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LRM38_RS10505 Protein ID WP_002892066.1
Coordinates 2059403..2059777 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM38_RS10495 (LRM38_10485) 2054555..2055748 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LRM38_RS10500 (LRM38_10490) 2055771..2058917 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LRM38_RS10505 (LRM38_10495) 2059403..2059777 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LRM38_RS10510 (LRM38_10500) 2059804..2060022 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LRM38_RS10515 (LRM38_10505) 2060181..2060747 + 567 WP_002892030.1 maltose O-acetyltransferase -
LRM38_RS10520 (LRM38_10510) 2060719..2060859 - 141 WP_004147370.1 hypothetical protein -
LRM38_RS10525 (LRM38_10515) 2060880..2061350 + 471 WP_002892026.1 YlaC family protein -
LRM38_RS10530 (LRM38_10520) 2061325..2062776 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LRM38_RS10535 (LRM38_10525) 2062877..2063575 + 699 WP_002892021.1 GNAT family protein -
LRM38_RS10540 (LRM38_10530) 2063572..2063712 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LRM38_RS10545 (LRM38_10535) 2063712..2063975 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T229964 WP_002892050.1 NZ_CP090061:2059804-2060022 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T229964 NZ_CP124651:c5720351-5720061 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT229964 WP_002892066.1 NZ_CP090061:2059403-2059777 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT229964 NZ_CP124651:c5720647-5720348 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References