Detailed information of TA system TA229945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3788651..3790145
• Replicon: chromosome
• Accession: NZ_CP090059
• Organism: Sphingomonas cannabina strain DM2-R-LB4

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LZK98_RS17740
• Protein ID: WP_233783843.1
• Coordinates: 3788651..3789535 (-)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LZK98_RS17745
• Protein ID: WP_233783844.1
• Coordinates: 3789528..3790145 (-)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK98_RS17740 (LZK98_17740)	3788651..3789535	-	885	WP_233783843.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LZK98_RS17745 (LZK98_17745)	3789528..3790145	-	618	WP_233783844.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LZK98_RS17750 (LZK98_17750)	3790191..3793283	-	3093	WP_233783845.1	helicase-related protein	-
LZK98_RS17755 (LZK98_17755)	3793280..3795130	-	1851	WP_233783846.1	phospholipase D family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-264)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (18-56)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 32641.25 Da
• Isoelectric Point: 5.0830

>T229945 WP_233783843.1 NZ_CP090059:c3789535-3788651 [Sphingomonas cannabina]
MAKEIKNIGASVRARLLQVAKASGQSFDLVLTRFALERLLFRLSQSPHADRFVLKGAMLMMSWFEDPYRGTRDLDLLGFG
DPEPEAMLATFRGILALNDDDGAEFDVDALRVDRIREELEYGGLRLRTTASISGARINLTIDIGFGDAMEPGAEMLDYPS
MLAFPAPRLRAYARETVIAEKFQAMVALGRANSRMKDFYDIWILSKSFDFAGDRLARAIAATFARRETAVPVDLPDALTP
AFANDEQKQRQWNAFVRDVSANPGSLEDVVNSLADFLMPHAIQARASNNNGSKL

Nucleotide

• Length: 885 bp

>T229945 NZ_CP124648:c868351-868004 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCTGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGCGGCAGTGATCTTGGTTCTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22338.87 Da
• Isoelectric Point: 10.4491

>AT229945 WP_233783844.1 NZ_CP090059:c3790145-3789528 [Sphingomonas cannabina]
MPETASQRHIAHSVLKARGIARLAELRTAGVTAATMSRMERDGEVLRLARGLYQLPDAPLDAHHSLAEAAKRVPKGVVCL
VSALAFHQLTDQLPRQVWIAIGQKDWAPKGDGAPVRLVRFTDRLLTEGVESHTVEGVPVKIFGVAKTVADCFRYRNKIGL
SVAIEGLQEALRQRKTTPGEIVRQAERGAIATVIRPYLEALTANG

Nucleotide

• Length: 618 bp

>AT229945 NZ_CP124648:c868612-868361 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA