Detailed information of TA system TA229943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-tacA/DUF1778(antitoxin)
• Location: 948970..949787
• Replicon: chromosome
• Accession: NZ_CP090059
• Organism: Sphingomonas cannabina strain DM2-R-LB4

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LZK98_RS04705
• Protein ID: WP_233785247.1
• Coordinates: 949269..949787 (+)
• Length: 173 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: LZK98_RS04700
• Protein ID: WP_233785246.1
• Coordinates: 948970..949254 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK98_RS04670 (LZK98_04670)	944090..945283	+	1194	WP_233785240.1	ThiF family adenylyltransferase	-
LZK98_RS04675 (LZK98_04675)	945419..946168	+	750	WP_233785241.1	hypothetical protein	-
LZK98_RS04680 (LZK98_04680)	946363..947475	-	1113	WP_233785242.1	macro domain-containing protein	-
LZK98_RS04685 (LZK98_04685)	947720..948082	-	363	WP_233785243.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
LZK98_RS04690 (LZK98_04690)	948237..948482	+	246	WP_233785244.1	DUF2188 domain-containing protein	-
LZK98_RS04695 (LZK98_04695)	948594..948791	+	198	WP_233785245.1	hypothetical protein	-
LZK98_RS04700 (LZK98_04700)	948970..949254	+	285	WP_233785246.1	DUF1778 domain-containing protein	Antitoxin
LZK98_RS04705 (LZK98_04705)	949269..949787	+	519	WP_233785247.1	GNAT family N-acetyltransferase	Toxin
LZK98_RS04710 (LZK98_04710)	950024..952000	-	1977	WP_233785248.1	relaxase/mobilization nuclease RlxS	-
LZK98_RS04715 (LZK98_04715)	952349..952669	+	321	WP_233785249.1	DUF1905 domain-containing protein	-
LZK98_RS04720 (LZK98_04720)	952770..953129	-	360	WP_233785250.1	hypothetical protein	-
LZK98_RS04725 (LZK98_04725)	953381..954184	-	804	WP_233785251.1	exodeoxyribonuclease III	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 173 a.a.
• Molecular weight: 18525.21 Da
• Isoelectric Point: 9.6228

>T229943 WP_233785247.1 NZ_CP090059:949269-949787 [Sphingomonas cannabina]
MSRRPYAAPTPITADHRLDGFACGKSPLDDWLRAHALENEGKASRTYVVTAQAGEDAGNVVAYYTLAYGSVTREEVPKKI
RQGLPNPVPVMVLGRLAVHRNHGGKGIGAGLLREAMQRTLDASLVAGLRALIVHALDDEAVGFYARYGFQVFPAGTRTLF
LPVETLRQAVAG

Nucleotide

• Length: 519 bp

>T229943 NZ_CP124646:6710742-6711119 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10485.02 Da
• Isoelectric Point: 4.8605

>AT229943 WP_233785246.1 NZ_CP090059:948970-949254 [Sphingomonas cannabina]
MADVLERPKADTTITMRLPVQTRELIDRAAATIDKSRTEFVLESARQRAIDVLLDQRVFHLDAAASEAFADILEKPPAPV
EALRTLVASKSPWE

Nucleotide

• Length: 285 bp

>AT229943 NZ_CP124646:6711100-6711435 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA