Detailed information of TA system TA229942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 712596..714072
• Replicon: chromosome
• Accession: NZ_CP090059
• Organism: Sphingomonas cannabina strain DM2-R-LB4

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: LZK98_RS03550
• Protein ID: WP_233784994.1
• Coordinates: 713209..714072 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: LZK98_RS03545
• Protein ID: WP_233784992.1
• Coordinates: 712596..713216 (+)
• Length: 207 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZK98_RS03525 (LZK98_03525)	708798..709835	-	1038	WP_233784988.1	DUF1254 domain-containing protein	-
LZK98_RS03530 (LZK98_03530)	710022..710921	+	900	WP_233784990.1	LysR family transcriptional regulator	-
LZK98_RS03535 (LZK98_03535)	711394..711993	-	600	Protein_700	recombinase family protein	-
LZK98_RS03540 (LZK98_03540)	712155..712493	+	339	WP_233784991.1	hypothetical protein	-
LZK98_RS03545 (LZK98_03545)	712596..713216	+	621	WP_233784992.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
LZK98_RS03550 (LZK98_03550)	713209..714072	+	864	WP_233784994.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LZK98_RS03555 (LZK98_03555)	714316..715170	+	855	WP_233784996.1	SMP-30/gluconolactonase/LRE family protein	-
LZK98_RS03560 (LZK98_03560)	715282..716217	-	936	WP_233784997.1	Gfo/Idh/MocA family oxidoreductase	-
LZK98_RS03565 (LZK98_03565)	716332..717480	+	1149	WP_233784998.1	aldose epimerase family protein	-
LZK98_RS03570 (LZK98_03570)	717482..718216	-	735	WP_233785000.1	FadR/GntR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-263)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-57)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32017.65 Da
• Isoelectric Point: 4.8802

>T229942 WP_233784994.1 NZ_CP090059:713209-714072 [Sphingomonas cannabina]
MASERANRAASVKDRLLNLARRDGRVFDVVLVRYALERLLYRLSLSKHRDRFVLKGGMLVTIWLPGGNRETRDADFLGYG
DASEATIRATFAEIMAIASDDGLRFDIDNMTVATIRDDIEYGGIRLRVSAYLERTRIPMVVDIGFGDALPTPPGELDYPT
LLDMEVPRIRSYPPVTVIAEKFQAMVQLGIGNGRMKDYYDLWALPQAMPIAADELDAAIRATFERRGTDIPRERPPGLSP
ELADDETKQRQWRAYAASIELEGVSLAEVVEAIWALVGPSCQRLAGS

Nucleotide

• Length: 864 bp

>T229942 NZ_CP124646:6698311-6698610 [Pseudomonas aeruginosa]
ATGAAAGAGCAATTTTACGACTACGACCCCGCTGATGCGTTGACTGATGCGGAGTCCATCGCGGTATTCATGGAGGACGC
ATTCGAAACGGGCGATGCGGCCTATATCGCAAAATGCCTGGGTGTCGTAGCCCGCGCCAAAGGGATGACCGCCCTTGCCA
ATGAATCTGGCCTTTCCCGCGAACACCTCTACCGGTCGTTCTCGGACAAGGGCAACCCGACTTTGAAAACCCTGCTGGCC
GTGATGCGTGCCGTTGGCCTGGATCTTACGACCCGCAAGCACGTTGAGCCGGTGCACTAA

Antitoxin

Protein

• Length: 207 a.a.
• Molecular weight: 22417.93 Da
• Isoelectric Point: 10.6619

>AT229942 WP_233784992.1 NZ_CP090059:712596-713216 [Sphingomonas cannabina]
MAPVPETQRDRLRDLLVQHPVARAQELRNAGIAAATIARAVKDGEVERVGRGLYQRPDAAIDAAHALAETAKRIPKGVIA
MVSALAFHGLTDQMPRKVWVAIGPGDWSPVSSYPPTRIVRFSEKYLREGVEHHMISGVSVPIYSIPKTLADLFRNSKLVD
RSVAVEGLRAALDQRKATPAAIAQAAKAGGAWRIMRPYLEALTFNG

Nucleotide

• Length: 621 bp

>AT229942 NZ_CP124646:6698012-6698314 [Pseudomonas aeruginosa]
ATGAAGACAATTAAACAAACCGCCACGTACAGGAACTGGGAACGCAAGCTGCGAGACAAGCAGGCCAAAGCCATCATCGC
TGCGCGAGTCTTCCGCGTTGCGCATGGTCTGCTTGGAGACGTTCAACCGGTGGGCCAAGGAATCAGCGAATTACGCATCC
ATCACGGCCCCGGCTACCGGGTGTATTTTCAGCAGCGAGGTAACCAGCTGGTCCTGTTGCTGTGTGGTGGAGACAAGAGC
AGCCAAGCGCGGGATATCGAAACCGCCAAAGCCTTGGCCAGCCAGTGGAGTGACGATGAATGA