Detailed information of TA system TA229941

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 883254..883847
• Replicon: chromosome
• Accession: NZ_CP090029
• Organism: Pseudomonas sivasensis strain BsEB-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LRO94_RS03800
• Protein ID: WP_032890654.1
• Coordinates: 883548..883847 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A031IS10
• Locus tag: LRO94_RS03795
• Protein ID: WP_032890601.1
• Coordinates: 883254..883544 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRO94_RS03775	879646..881712	-	2067	WP_179179577.1	TonB-dependent copper receptor	-
LRO94_RS03780	881806..882219	-	414	WP_095186040.1	DUF2946 domain-containing protein	-
LRO94_RS03785	882231..882713	-	483	WP_179179576.1	copper chaperone PCu(A)C	-
LRO94_RS03790	882761..883156	-	396	WP_095186039.1	DUF2946 domain-containing protein	-
LRO94_RS03795	883254..883544	-	291	WP_032890601.1	putative addiction module antidote protein	Antitoxin
LRO94_RS03800	883548..883847	-	300	WP_032890654.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRO94_RS03805	884369..884620	+	252	WP_032890602.1	DUF6124 family protein	-
LRO94_RS03810	884746..885459	-	714	WP_179179575.1	cobalt-precorrin-6A reductase	-
LRO94_RS03815	885456..886661	-	1206	WP_179179574.1	precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE	-
LRO94_RS03820	886808..888046	+	1239	WP_233897052.1	precorrin-3B synthase	-
LRO94_RS03825	888039..888665	+	627	WP_032890608.1	precorrin-8X methylmutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (6-95)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11482.08 Da
• Isoelectric Point: 6.9822

>T229941 WP_032890654.1 NZ_CP090029:c883847-883548 [Pseudomonas sivasensis]
IEFNETTEFVKWLEAVRDPFVKVRVIKRVRMAEAGNFGDCESVGDSVFEMRIHYGPGYRVYFTRRDEVVYLLLIGGDKST
QSRDIKRAKQIAINFGSEE

Nucleotide

• Length: 300 bp

>T229941 NZ_CP124646:6698012-6698314 [Pseudomonas aeruginosa]
ATGAAGACAATTAAACAAACCGCCACGTACAGGAACTGGGAACGCAAGCTGCGAGACAAGCAGGCCAAAGCCATCATCGC
TGCGCGAGTCTTCCGCGTTGCGCATGGTCTGCTTGGAGACGTTCAACCGGTGGGCCAAGGAATCAGCGAATTACGCATCC
ATCACGGCCCCGGCTACCGGGTGTATTTTCAGCAGCGAGGTAACCAGCTGGTCCTGTTGCTGTGTGGTGGAGACAAGAGC
AGCCAAGCGCGGGATATCGAAACCGCCAAAGCCTTGGCCAGCCAGTGGAGTGACGATGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10417.99 Da
• Isoelectric Point: 5.8124

>AT229941 WP_032890601.1 NZ_CP090029:c883544-883254 [Pseudomonas sivasensis]
MSESFTRFDAAKYLKTPLEMAAYLETCFEDDAGDGLLIRTALNDIARAQGMTQVARDAGLGRESLYKALSSNGNPEFATI
MKVMKALGLKLHATAI

Nucleotide

• Length: 291 bp

>AT229941 NZ_CP124646:6698311-6698610 [Pseudomonas aeruginosa]
ATGAAAGAGCAATTTTACGACTACGACCCCGCTGATGCGTTGACTGATGCGGAGTCCATCGCGGTATTCATGGAGGACGC
ATTCGAAACGGGCGATGCGGCCTATATCGCAAAATGCCTGGGTGTCGTAGCCCGCGCCAAAGGGATGACCGCCCTTGCCA
ATGAATCTGGCCTTTCCCGCGAACACCTCTACCGGTCGTTCTCGGACAAGGGCAACCCGACTTTGAAAACCCTGCTGGCC
GTGATGCGTGCCGTTGGCCTGGATCTTACGACCCGCAAGCACGTTGAGCCGGTGCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A031IS10