Detailed information of TA system TA229914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 460615..461263
• Replicon: chromosome
• Accession: NZ_CP090006
• Organism: Virgibacillus halodenitrificans strain ASH15

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A6L8JWB3
• Locus tag: LWC06_RS02420
• Protein ID: WP_019379217.1
• Coordinates: 460904..461263 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LWC06_RS02415
• Protein ID: WP_233880187.1
• Coordinates: 460615..460899 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LWC06_RS02395	456100..456459	+	360	WP_233880183.1	holo-ACP synthase	-
LWC06_RS02400	456519..458039	+	1521	WP_233880184.1	NAD(P)H-hydrate dehydratase	-
LWC06_RS02405	458127..459137	+	1011	WP_233880185.1	outer membrane lipoprotein carrier protein LolA	-
LWC06_RS02410	459328..460452	+	1125	WP_233880186.1	alanine racemase	-
LWC06_RS02415	460615..460899	+	285	WP_233880187.1	antitoxin	Antitoxin
LWC06_RS02420	460904..461263	+	360	WP_019379217.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LWC06_RS02425	461624..462493	+	870	WP_233880189.1	RsbT co-antagonist protein RsbRA	-
LWC06_RS02430	462499..462855	+	357	WP_040954281.1	STAS domain-containing protein	-
LWC06_RS02435	462858..463259	+	402	WP_233880190.1	anti-sigma regulatory factor	-
LWC06_RS02440	463367..464380	+	1014	WP_233880191.1	PP2C family protein-serine/threonine phosphatase	-
LWC06_RS02445	464487..464819	+	333	WP_060681899.1	STAS domain-containing protein	-
LWC06_RS02450	464819..465295	+	477	WP_019379211.1	anti-sigma B factor RsbW	-
LWC06_RS02455	465267..466058	+	792	WP_060681900.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13377.53 Da
• Isoelectric Point: 8.4940

>T229914 WP_019379217.1 NZ_CP090006:460904-461263 [Virgibacillus halodenitrificans]
MIVQRGEVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRNSVILLEQI
RTLDKQRLTDKITKLDAEMMNKINQALEISLGLKDMYDH

Nucleotide

• Length: 360 bp

>T229914 NZ_CP124632:c6359755-6359477 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10888.19 Da
• Isoelectric Point: 4.2057

>AT229914 WP_233880187.1 NZ_CP090006:460615-460899 [Virgibacillus halodenitrificans]
MSESLQEILVKIPKNLLNEVDGLMRYENSDLSDFICQATRSYLQDKKDEHIQQFRETMQKGYEEMGRINLTIASEAFQAE
EEAENTLERSVIGV

Nucleotide

• Length: 285 bp

>AT229914 NZ_CP124632:c6359465-6359160 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAATGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Structures

Toxin

• AlphaFold DB: A0A6L8JWB3