Detailed information of TA system TA229908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 820062..820648
• Replicon: chromosome
• Accession: NZ_CP090004
• Organism: Flagellimonas sp. HMM57

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LV716_RS03625
• Protein ID: WP_163416428.1
• Coordinates: 820062..820367 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LV716_RS03630
• Protein ID: WP_163416429.1
• Coordinates: 820364..820648 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV716_RS03595 (LV716_03595)	815847..816476	+	630	WP_163416423.1	bifunctional nuclease family protein	-
LV716_RS03600 (LV716_03600)	816486..817991	+	1506	WP_163416424.1	nucleoside transporter C-terminal domain-containing protein	-
LV716_RS03605 (LV716_03605)	818107..818466	+	360	WP_163416425.1	four helix bundle protein	-
LV716_RS03610 (LV716_03610)	818509..819333	+	825	WP_163416426.1	thymidylate synthase	-
LV716_RS03615 (LV716_03615)	819511..819705	-	195	WP_163416029.1	hypothetical protein	-
LV716_RS03620 (LV716_03620)	819760..819984	+	225	WP_163416427.1	helix-turn-helix transcriptional regulator	-
LV716_RS03625 (LV716_03625)	820062..820367	+	306	WP_163416428.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LV716_RS03630 (LV716_03630)	820364..820648	+	285	WP_163416429.1	putative addiction module antidote protein	Antitoxin
LV716_RS03635 (LV716_03635)	820645..821181	+	537	WP_163416430.1	nuclear transport factor 2 family protein	-
LV716_RS03640 (LV716_03640)	821269..821769	+	501	WP_163416431.1	hypothetical protein	-
LV716_RS03645 (LV716_03645)	821832..822188	+	357	WP_163416432.1	cupin domain-containing protein	-
LV716_RS03650 (LV716_03650)	822190..822612	+	423	WP_163416433.1	SRPBCC domain-containing protein	-
LV716_RS03655 (LV716_03655)	822716..824470	-	1755	WP_233759222.1	hypothetical protein	-
LV716_RS03660 (LV716_03660)	824698..825411	+	714	WP_163416434.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-88)

Antitoxin

• Uncharacterized protein HI1420 (2-89)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11823.14 Da
• Isoelectric Point: 10.8541

>T229908 WP_163416428.1 NZ_CP090004:820062-820367 [Flagellimonas sp. HMM57]
MFFIEKTIEFDKWFRKLKKLKAKSKILFRIQKIQSNGHFGDCKAVGDGIREKGVNFAKGYRVYFKEKKGKIVILLIGGDK
STQQKDILKAKDIWKNLIDKK

Nucleotide

• Length: 306 bp

>T229908 NZ_CP124632:3905658-3905948 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10084.63 Da
• Isoelectric Point: 5.8149

>AT229908 WP_163416429.1 NZ_CP090004:820364-820648 [Flagellimonas sp. HMM57]
MKTSKFDIADYLDSKEMIAEYLNTVLEEGDNADIINAIGHVAKAIGMTKISEETGLSRPSLYKALSDGAKPQFATVIKVL
KAIGGKIQVNPISS

Nucleotide

• Length: 285 bp

>AT229908 NZ_CP124632:3905362-3905661 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA