Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2073543..2074057 Replicon chromosome
Accession NZ_CP090001
Organism Staphylococcus aureus strain RN6390

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag LV622_RS10205 Protein ID WP_000581792.1
Coordinates 2073543..2073752 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag LV622_RS10210 Protein ID WP_000138932.1
Coordinates 2073764..2074057 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LV622_RS10185 (LV622_10185) 2069051..2070409 - 1359 WP_000611465.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
LV622_RS10190 (LV622_10190) 2070424..2071494 - 1071 WP_000159631.1 D-alanine--D-alanine ligase -
LV622_RS10195 (LV622_10195) 2071812..2073014 + 1203 WP_001109939.1 FtsW/RodA/SpoVE family cell cycle protein -
LV622_RS10200 (LV622_10200) 2073278..2073415 - 138 WP_000828354.1 Lmo0850 family protein -
LV622_RS10205 (LV622_10205) 2073543..2073752 - 210 WP_000581792.1 heavy metal-associated domain-containing protein Toxin
LV622_RS10210 (LV622_10210) 2073764..2074057 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
LV622_RS10215 (LV622_10215) 2074223..2075707 + 1485 WP_000571549.1 cardiolipin synthase -
LV622_RS10220 (LV622_10220) 2075735..2076382 + 648 WP_001187629.1 HD domain-containing protein -
LV622_RS10225 (LV622_10225) 2077195..2078067 - 873 WP_000725802.1 membrane protein insertase YidC -
LV622_RS10230 (LV622_10230) 2078153..2078794 - 642 WP_000483153.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T229903 WP_000581792.1 NZ_CP090001:c2073752-2073543 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T229903 NZ_CP124632:c478529-478248 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT229903 WP_000138932.1 NZ_CP090001:c2074057-2073764 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT229903 NZ_CP124632:c478753-478526 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References