Detailed information of TA system TA022989

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 2590409..2590940
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4SFA6
• Locus tag: PPHA_RS12345
• Protein ID: WP_012509159.1
• Coordinates: 2590659..2590940 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: B4SFA5
• Locus tag: PPHA_RS12340
• Protein ID: WP_012509158.1
• Coordinates: 2590409..2590672 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS12320	2586455..2587279	+	825	WP_041526892.1	FAD/NAD(P)-binding protein	-
PPHA_RS12325	2587281..2588057	+	777	WP_012509155.1	NADH ubiquinone oxidoreductase	-
PPHA_RS12330	2588047..2589321	+	1275	WP_012509156.1	Ni/Fe hydrogenase subunit alpha	-
PPHA_RS12335	2589662..2590168	+	507	WP_012509157.1	isoprenylcysteine carboxylmethyltransferase family protein	-
PPHA_RS12340	2590409..2590672	+	264	WP_012509158.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
PPHA_RS12345	2590659..2590940	+	282	WP_012509159.1	type II toxin-antitoxin system YafQ family toxin	Toxin
PPHA_RS15280	2591005..2591181	-	177	WP_083765354.1	tyrosine-type recombinase/integrase	-
PPHA_RS12350	2591345..2591737	-	393	WP_012509160.1	hypothetical protein	-
PPHA_RS12355	2592025..2592366	+	342	Protein_2437	DUF1778 domain-containing protein	-
PPHA_RS12360	2592363..2592584	+	222	Protein_2438	GNAT family N-acetyltransferase	-
PPHA_RS12365	2592702..2593019	+	318	WP_083765336.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPHA_RS12370	2593425..2594561	+	1137	WP_012509161.1	AAA family ATPase	-
PPHA_RS12375	2594554..2595228	+	675	WP_012509162.1	DUF4276 family protein	-
PPHA_RS12380	2595250..2595879	+	630	Protein_2442	DUF4143 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10682.34 Da
• Isoelectric Point: 7.3568

>T22989 WP_012509159.1 NC_011060:2590659-2590940 [Pelodictyon phaeoclathratiforme BU-1]
MRRIELSRKFRHDYRREKSGMYGKSLDALLNGILDLLESDEVLPPNAVDHALMGKFTGYRDCHIKPDLILIYRKTGDDVL
ELVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T22989 NC_011060:2590659-2590940 [Pelodictyon phaeoclathratiforme BU-1]
ATGAGGAGGATTGAGCTATCCCGGAAATTCAGGCATGACTATCGAAGAGAAAAATCAGGAATGTATGGGAAATCCCTTGA
TGCTCTATTAAATGGAATCCTTGATCTCCTCGAGTCCGATGAGGTCTTGCCGCCCAATGCCGTCGATCATGCGCTTATGG
GAAAATTCACAGGGTACCGCGATTGCCATATCAAGCCTGATCTTATTTTGATCTACCGCAAAACAGGCGACGACGTTCTT
GAGCTGGTTCGTCTTGGATCACACAGTGAACTCGGATTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9508.05 Da
• Isoelectric Point: 4.3700

>AT22989 WP_012509158.1 NC_011060:2590409-2590672 [Pelodictyon phaeoclathratiforme BU-1]
MIANTVVRARIDEKTKAEATAVLAAIGLTLSDAVRLMLMRVAAEKALPFDPLIPNAETIEAIMESQRGELIAVGSREELF
RVLNEED

Nucleotide

• Length: 264 bp

>AT22989 NC_011060:2590409-2590672 [Pelodictyon phaeoclathratiforme BU-1]
ATGATTGCAAACACTGTTGTGAGGGCGCGAATCGACGAAAAAACCAAAGCTGAAGCCACCGCAGTACTGGCTGCCATAGG
GCTCACCCTGTCCGATGCCGTGCGCCTTATGCTGATGCGGGTTGCCGCTGAAAAAGCACTGCCATTTGATCCGCTCATTC
CGAATGCCGAGACGATAGAAGCTATTATGGAGTCGCAACGAGGAGAGCTTATCGCCGTTGGTTCCAGAGAAGAATTATTC
AGGGTGCTTAATGAGGAGGATTGA

Structures

Toxin

• AlphaFold DB: B4SFA6

Antitoxin

• AlphaFold DB: B4SFA5