Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4935150..4935792 Replicon chromosome
Accession NZ_CP089993
Organism Bacillus anthracis strain BF5

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag LKW29_RS25835 Protein ID WP_000635963.1
Coordinates 4935442..4935792 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag LKW29_RS25830 Protein ID WP_000004570.1
Coordinates 4935150..4935437 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKW29_RS25805 (LKW29_25805) 4930466..4931428 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
LKW29_RS25810 (LKW29_25810) 4931421..4931993 - 573 WP_000906915.1 rhomboid family intramembrane serine protease -
LKW29_RS25815 (LKW29_25815) 4932086..4932445 + 360 WP_000635040.1 holo-ACP synthase -
LKW29_RS25820 (LKW29_25820) 4932602..4933552 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
LKW29_RS25825 (LKW29_25825) 4933671..4934840 + 1170 WP_000390596.1 alanine racemase -
LKW29_RS25830 (LKW29_25830) 4935150..4935437 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
LKW29_RS25835 (LKW29_25835) 4935442..4935792 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
LKW29_RS25840 (LKW29_25840) 4935860..4938028 + 2169 WP_000426225.1 Tex family protein -
LKW29_RS25845 (LKW29_25845) 4938086..4938202 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
LKW29_RS25850 (LKW29_25850) 4938398..4938856 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T229885 WP_000635963.1 NZ_CP089993:4935442-4935792 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T229885 NZ_CP124624:c148121-147840 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT229885 WP_000004570.1 NZ_CP089993:4935150-4935437 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT229885 NZ_CP124624:c148345-148118 [Pseudomonas aeruginosa]
ATGAGCACTGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References