Detailed information of TA system TA229883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6104..6684
• Replicon: plasmid pZR1
• Accession: NZ_CP089992
• Organism: Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: LUR00_RS27010
• Protein ID: WP_071177730.1
• Coordinates: 6104..6418 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: LUR00_RS27015
• Protein ID: WP_000093040.1
• Coordinates: 6406..6684 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUR00_RS26985 (LUR00_26985)	2048..4012	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
LUR00_RS26990 (LUR00_26990)	4012..4743	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
LUR00_RS26995 (LUR00_26995)	4750..5280	+	531	WP_071177729.1	hypothetical protein	-
LUR00_RS27000 (LUR00_27000)	5307..5486	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
LUR00_RS27005 (LUR00_27005)	5512..5940	-	429	WP_001140599.1	hypothetical protein	-
LUR00_RS27010 (LUR00_27010)	6104..6418	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUR00_RS27015 (LUR00_27015)	6406..6684	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
LUR00_RS27020 (LUR00_27020)	6859..7224	-	366	WP_072354022.1	TonB family protein	-
LUR00_RS27025 (LUR00_27025)	7221..7592	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
LUR00_RS27030 (LUR00_27030)	7866..8111	-	246	WP_032440458.1	hypothetical protein	-
LUR00_RS27035 (LUR00_27035)	8756..10243	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T229883 WP_071177730.1 NZ_CP089992:c6418-6104 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T229883 NZ_CP124622:c4982404-4982057 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT229883 WP_000093040.1 NZ_CP089992:c6684-6406 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT229883 NZ_CP124622:c4982665-4982414 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTATTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1