Detailed information of TA system TA229880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 53639..54549
• Replicon: plasmid p3IncF
• Accession: NZ_CP089990
• Organism: Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: LUR00_RS26015
• Protein ID: WP_004144067.1
• Coordinates: 54079..54549 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6A8PHD0
• Locus tag: LUR00_RS26010
• Protein ID: WP_023287184.1
• Coordinates: 53639..54082 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUR00_RS25990 (LUR00_25990)	49055..49804	+	750	Protein_53	IS5 family transposase	-
LUR00_RS25995 (LUR00_25995)	49833..50801	+	969	WP_000654811.1	IS5 family transposase	-
LUR00_RS26000 (LUR00_26000)	50997..52343	+	1347	WP_225517490.1	ThiF family adenylyltransferase	-
LUR00_RS26005 (LUR00_26005)	52396..53538	+	1143	Protein_56	translesion error-prone DNA polymerase V subunit UmuC	-
LUR00_RS26010 (LUR00_26010)	53639..54082	+	444	WP_023287184.1	DUF2384 domain-containing protein	Antitoxin
LUR00_RS26015 (LUR00_26015)	54079..54549	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
LUR00_RS26020 (LUR00_26020)	54636..55631	+	996	WP_077254794.1	IS5 family transposase	-
LUR00_RS26025 (LUR00_26025)	55633..56601	+	969	WP_072096747.1	IS5 family transposase	-
LUR00_RS26030 (LUR00_26030)	56790..57050	-	261	WP_017901409.1	hypothetical protein	-
LUR00_RS26035 (LUR00_26035)	57355..58323	+	969	WP_074423483.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / sul1 / blaDHA-1 / qnrB4	-	1..190701	190701
	-	inside	IScluster/Tn	-	-	46911..58323	11412

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T229880 WP_004144067.1 NZ_CP089990:54079-54549 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T229880 NZ_CP089990:54079-54549 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACAGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16522.84 Da
• Isoelectric Point: 10.3013

>AT229880 WP_023287184.1 NZ_CP089990:53639-54082 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT229880 NZ_CP089990:53639-54082 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAAGTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACTGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6A8PHD0