Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2107505..2108130 Replicon chromosome
Accession NZ_CP089989
Organism Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LUR00_RS10040 Protein ID WP_002882817.1
Coordinates 2107505..2107888 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LUR00_RS10045 Protein ID WP_004150355.1
Coordinates 2107888..2108130 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUR00_RS10025 (LUR00_10025) 2104871..2105773 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LUR00_RS10030 (LUR00_10030) 2105770..2106405 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LUR00_RS10035 (LUR00_10035) 2106402..2107331 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LUR00_RS10040 (LUR00_10040) 2107505..2107888 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LUR00_RS10045 (LUR00_10045) 2107888..2108130 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LUR00_RS10050 (LUR00_10050) 2108335..2109252 + 918 WP_021466676.1 alpha/beta hydrolase -
LUR00_RS10055 (LUR00_10055) 2109266..2110207 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LUR00_RS10060 (LUR00_10060) 2110252..2110689 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LUR00_RS10065 (LUR00_10065) 2110686..2111546 - 861 WP_004146232.1 virulence factor BrkB family protein -
LUR00_RS10070 (LUR00_10070) 2111540..2112139 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229867 WP_002882817.1 NZ_CP089989:c2107888-2107505 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229867 NZ_CP124600:c5861369-5861091 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229867 WP_004150355.1 NZ_CP089989:c2108130-2107888 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229867 NZ_CP124600:c5861079-5860774 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References