Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5201075..5201700 Replicon chromosome
Accession NZ_CP089986
Organism Klebsiella pneumoniae strain LZKP00001

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LUA09_RS25055 Protein ID WP_002882817.1
Coordinates 5201075..5201458 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LUA09_RS25060 Protein ID WP_004150355.1
Coordinates 5201458..5201700 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUA09_RS25040 (LUA09_25040) 5198441..5199343 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LUA09_RS25045 (LUA09_25045) 5199340..5199975 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LUA09_RS25050 (LUA09_25050) 5199972..5200901 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LUA09_RS25055 (LUA09_25055) 5201075..5201458 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LUA09_RS25060 (LUA09_25060) 5201458..5201700 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LUA09_RS25065 (LUA09_25065) 5201905..5202822 + 918 WP_004895173.1 alpha/beta hydrolase -
LUA09_RS25070 (LUA09_25070) 5202836..5203777 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LUA09_RS25075 (LUA09_25075) 5203822..5204259 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LUA09_RS25080 (LUA09_25080) 5204256..5205116 - 861 WP_004146232.1 virulence factor BrkB family protein -
LUA09_RS25085 (LUA09_25085) 5205110..5205709 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T229861 WP_002882817.1 NZ_CP089986:c5201458-5201075 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T229861 NZ_CP124600:1756810-1757565 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCTACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT229861 WP_004150355.1 NZ_CP089986:c5201700-5201458 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT229861 NZ_CP124600:1756307-1756675 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References