Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4624754..4625564 Replicon chromosome
Accession NZ_CP089986
Organism Klebsiella pneumoniae strain LZKP00001

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LUA09_RS22340 Protein ID WP_004178461.1
Coordinates 4624754..4625287 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LUA09_RS22345 Protein ID WP_002887278.1
Coordinates 4625298..4625564 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUA09_RS22335 (LUA09_22335) 4623585..4624706 + 1122 WP_002887282.1 cupin domain-containing protein -
LUA09_RS22340 (LUA09_22340) 4624754..4625287 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LUA09_RS22345 (LUA09_22345) 4625298..4625564 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LUA09_RS22350 (LUA09_22350) 4625667..4627100 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
LUA09_RS22355 (LUA09_22355) 4627090..4627773 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LUA09_RS22360 (LUA09_22360) 4627945..4629330 + 1386 WP_032439492.1 efflux transporter outer membrane subunit -
LUA09_RS22365 (LUA09_22365) 4629348..4629692 + 345 WP_004894569.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T229859 WP_004178461.1 NZ_CP089986:c4625287-4624754 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T229859 NZ_CP124600:c147752-147471 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT229859 WP_002887278.1 NZ_CP089986:c4625564-4625298 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT229859 NZ_CP124600:c147976-147749 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References