Detailed information of TA system TA229843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4868469..4869105
• Replicon: chromosome
• Accession: NZ_CP089985
• Organism: Pseudomonas sp. HN11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3M3WYJ9
• Locus tag: LVW35_RS22165
• Protein ID: WP_043205781.1
• Coordinates: 4868469..4868873 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LVW35_RS22170
• Protein ID: WP_080589294.1
• Coordinates: 4868866..4869105 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVW35_RS22135 (LVW35_22135)	4864697..4864966	+	270	WP_233892038.1	DUF2790 domain-containing protein	-
LVW35_RS22140 (LVW35_22140)	4865114..4865527	-	414	WP_233892039.1	type II toxin-antitoxin system VapC family toxin	-
LVW35_RS22145 (LVW35_22145)	4865524..4865754	-	231	WP_233892040.1	type II toxin-antitoxin system VapB family antitoxin	-
LVW35_RS22150 (LVW35_22150)	4866239..4867489	+	1251	WP_233892041.1	ribonucleotide-diphosphate reductase subunit beta	-
LVW35_RS22155 (LVW35_22155)	4867579..4867725	+	147	Protein_4368	HNH endonuclease	-
LVW35_RS22160 (LVW35_22160)	4867798..4868442	+	645	WP_122728393.1	HAD-IB family hydrolase	-
LVW35_RS22165 (LVW35_22165)	4868469..4868873	-	405	WP_043205781.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVW35_RS22170 (LVW35_22170)	4868866..4869105	-	240	WP_080589294.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVW35_RS22175 (LVW35_22175)	4869479..4870438	-	960	WP_233896534.1	LysR family transcriptional regulator	-
LVW35_RS22180 (LVW35_22180)	4870632..4872059	+	1428	WP_233892042.1	argininosuccinate lyase	-
LVW35_RS22185 (LVW35_22185)	4872167..4873045	+	879	WP_233892043.1	amino acid ABC transporter permease	-
LVW35_RS22190 (LVW35_22190)	4873070..4873834	+	765	WP_010208290.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14987.25 Da
• Isoelectric Point: 7.4002

>T229843 WP_043205781.1 NZ_CP089985:c4868873-4868469 [Pseudomonas sp. HN11]
MIKYMLDTNIVIYIIKRRPIEILEKFNANVGRMVISSITLAELMHGAEKSQLVEKNTRAVEDFSSRLDVLSYDDKAAFHY
GSIRSDLEKKGTPIGVNDLHIAGHARSSGLVLVTNNEGEFKRVNGLIVENWIGA

Nucleotide

• Length: 405 bp

>T229843 NZ_CP124543:5512643-5512984 [Halotia branconii CENA392]
ATGACAACCATTTGGATTGATGTACATTTGTCACCTGCGATCGCATCTTGGATTACAAATACCTTTGGGGTAACAGCAAT
AGCTCTACGCGATCTTGGACTGCGAGATGCCGAAGATCCTAAAATCTTTGAGGCTGCCAAAGCTGAAAGAGCTATATTTA
TGACCAAAGACAGTGATTTTGTGGATTTGGTTGATCGACTTGGAGTACCACCACAAATCATTTGGTTAACTTGCGGCAAC
ACCTCGAATGCTCGGTTGCAAGAAATTTTAGCTTCAACATTATTAGAAGCATTAGCGCTATTAAGTACTGGGGAACAATT
GGTAGAAATTAGCGGCGATTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9031.93 Da
• Isoelectric Point: 4.1068

>AT229843 WP_080589294.1 NZ_CP089985:c4869105-4868866 [Pseudomonas sp. HN11]
MSSGTIFTNNRTQNIRIPADMRFPAGVKTVEVRAVGRELIISPVENTWDSFFLASESVSEDFMEERADQTQQERESFDD

Nucleotide

• Length: 240 bp

>AT229843 NZ_CP124543:5512422-5512646 [Halotia branconii CENA392]
ATGTCAAACCTACTTGAACGAATTACAGTAAATCCTAAACAGTGTGGGGGTCGTCCTTGTATTCGTGGTATGAGGATTCG
AGTTTCTGATGTTCTAGATCTGTTTGCAGCAGGATTGAGTGCTGAGGAAATTTTAGAAGACCTACCAGATTTGGAAATGG
ATGACATAAGAGCATCACTAGTGTACGCAGCACGTAAACTAAACCATCCTGTGTTGGTTGCATGA

Structures

Toxin

• AlphaFold DB: A0A3M3WYJ9