Detailed information of TA system TA229839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1855259..1855863
• Replicon: chromosome
• Accession: NZ_CP089985
• Organism: Pseudomonas sp. HN11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A1S2UAI9
• Locus tag: LVW35_RS08580
• Protein ID: WP_065901605.1
• Coordinates: 1855259..1855447 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LVW35_RS08585
• Protein ID: WP_233894811.1
• Coordinates: 1855459..1855863 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVW35_RS08550 (LVW35_08550)	1850623..1851096	+	474	WP_233894805.1	cytochrome c-type biogenesis protein CcmH	-
LVW35_RS08555 (LVW35_08555)	1851089..1852273	+	1185	WP_233894806.1	c-type cytochrome biogenesis protein CcmI	-
LVW35_RS08560 (LVW35_08560)	1852289..1852684	+	396	WP_126571034.1	hypothetical protein	-
LVW35_RS08565 (LVW35_08565)	1852877..1853449	+	573	WP_233894807.1	phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN	-
LVW35_RS08570 (LVW35_08570)	1853440..1854189	+	750	WP_233894809.1	phosphonate metabolism protein PhnP	-
LVW35_RS08575 (LVW35_08575)	1854186..1855067	-	882	WP_233894810.1	LysR family transcriptional regulator	-
LVW35_RS08580 (LVW35_08580)	1855259..1855447	+	189	WP_065901605.1	type II toxin-antitoxin system HicA family toxin	Toxin
LVW35_RS08585 (LVW35_08585)	1855459..1855863	+	405	WP_233894811.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LVW35_RS08590 (LVW35_08590)	1856169..1857689	+	1521	WP_233894812.1	M10 family metallopeptidase C-terminal domain-containing protein	-
LVW35_RS08595 (LVW35_08595)	1857715..1858911	-	1197	WP_233894813.1	MFS transporter	-
LVW35_RS08600 (LVW35_08600)	1859083..1859697	+	615	WP_233894814.1	DUF5666 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7219.42 Da
• Isoelectric Point: 11.2000

>T229839 WP_065901605.1 NZ_CP089985:1855259-1855447 [Pseudomonas sp. HN11]
VQSRLLIKELQEAGWVLDRVTGSHHIFTHRYNPYTIPVPHPKKDLPLGTVRSIRKRAGLFQF

Nucleotide

• Length: 189 bp

>T229839 NZ_CP124543:3947821-3948075 [Halotia branconii CENA392]
ATGAAAGTGGTGACTTTTAGCGAAGCGAGAAGTAATCTCAAGACTGTTTTAGACCAAGTGGTGGAAGATGTCGACTACAC
CATCATTACTAGAAGAGATGCGGAGGATGCTGTAGTGATGTCGCTAGAGTTATTTAATAGTTTGCTAGAGACTGTCCATT
TACTCAAGTCTCCTGCCAATGCTGCTCATTTAGAGCGTTCTATTGCTCAGTTCAAGCAGGGGAAAGTGGTAGGGCAAAAT
TTGTTGAATGACTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14713.67 Da
• Isoelectric Point: 4.6000

>AT229839 WP_233894811.1 NZ_CP089985:1855459-1855863 [Pseudomonas sp. HN11]
MQYPICIEWGDDFTATGIQIPDIPGAVTAGDSFEEAYNAAVEVAHILLQEIAAEGGPIPMPTSVTNHHAHEDYAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHKVKSRSSFLADAALEKLVRS

Nucleotide

• Length: 405 bp

>AT229839 NZ_CP124543:3948068-3948334 [Halotia branconii CENA392]
ATGACTAGAAAGTTAGCATGGACAGATGAAGCTTGGAATGATTATCTATATTGGCAAGGGCAAGACAAGAAAATATTAAA
ACGAATTAACAAGCTAATTGAGGCAACAATCCAATTACCGTTTGAGGGAATTGGAAAACCAGAACTACTGAGGGAGAATT
TGGCTGGTTTTTGGTCGCGGCGAATCGATGATACGAACCGCTTAGTATATGCAGTAAATGACGAATATCTAACAATTATT
TCTTGTCGATACCATTATTTTGATTGA

Structures

Toxin

• AlphaFold DB: A0A1S2UAI9