Detailed information of TA system TA229822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 163033..163634
• Replicon: chromosome
• Accession: NZ_CP089973
• Organism: Pseudomonas brassicacearum strain WCS365

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: LRP86_RS00775
• Protein ID: WP_198286652.1
• Coordinates: 163033..163323 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1G4U731
• Locus tag: LRP86_RS00780
• Protein ID: WP_038858737.1
• Coordinates: 163326..163634 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRP86_RS00740	158527..158964	-	438	WP_233839792.1	GNAT family N-acetyltransferase	-
LRP86_RS00745 (LRP86_00149)	159028..159534	-	507	WP_100264118.1	AAA family ATPase	-
LRP86_RS00750 (LRP86_00150)	159835..160035	-	201	WP_013693816.1	hypothetical protein	-
LRP86_RS00755 (LRP86_00151)	160141..160566	-	426	WP_003203795.1	hypothetical protein	-
LRP86_RS00760 (LRP86_00152)	160820..161038	-	219	WP_100264119.1	hypothetical protein	-
LRP86_RS00765 (LRP86_00153)	161100..161645	-	546	WP_043042784.1	DUF4410 domain-containing protein	-
LRP86_RS00770 (LRP86_00154)	162337..162762	+	426	WP_233839793.1	hypothetical protein	-
LRP86_RS00775 (LRP86_00155)	163033..163323	+	291	WP_198286652.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRP86_RS00780 (LRP86_00156)	163326..163634	+	309	WP_038858737.1	putative addiction module antidote protein	Antitoxin
LRP86_RS00785 (LRP86_00157)	164320..164835	-	516	WP_233839794.1	hypothetical protein	-
LRP86_RS00790 (LRP86_00158)	165316..166185	+	870	WP_100264122.1	AraC family transcriptional regulator	-
LRP86_RS00795	166449..166772	-	324	WP_100264123.1	DUF2388 domain-containing protein	-
LRP86_RS00805 (LRP86_00160)	167164..168447	+	1284	WP_100264124.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	151430..166185	14755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10502.01 Da
• Isoelectric Point: 10.8582

>T229822 WP_198286652.1 NZ_CP089973:163033-163323 [Pseudomonas brassicacearum]
IYRIQQTQTFTAWHSSVRDLRAKNAIARRIDRAAAGNLGDVKPVGDGISELRVDVGAGYRDYFTMRGGLVIVLLAGGDKS
SQSADIRRAKKLAKEI

Nucleotide

• Length: 291 bp

>T229822 NZ_CP124527:24633-24887 [Psychrobacter sp. WB2]
TTGATACTATCTTGGACGGAAGACGCGTGGGAGGACTATGAGTACTGGCAAAGAACCAGTAAAGAGAAAGTCAAACAGAT
CGGTAAGCTGATTAAAGCCATCAAACGTGACCCATTTAAGGGTATTGGCAAACCTGAACCACTCAAACATGACCTTGCAG
GCTACTGGTCTCGTCGAATTGATCAAGAGCATCGGCTGGTTTATGAAATTCAAGATGATGCGATCATTATCGTCCAGTGC
CGTTATCATTACTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10928.34 Da
• Isoelectric Point: 5.9574

>AT229822 WP_038858737.1 NZ_CP089973:163326-163634 [Pseudomonas brassicacearum]
MSQHLTTFDMAALLDSDEAISEYLSQVLADGDSEEFLRAIGYVAKARGMAQIAKDSGMGRESLYKAFAPGSKPRFDTVLK
VIHALGIDLHAQPGHAANHSPA

Nucleotide

• Length: 309 bp

>AT229822 NZ_CP124527:24346-24636 [Psychrobacter sp. WB2]
ATGGACGTTGTGAACTACACTGATTTTAGAAAGAGCTTAGCAAAGTATATCGATAAGGTAGACACTGATAAAGGCCCGAT
ATTAATTACAAGACAAAATGCTAAACCGACGGTATTAATGAGTTTAGAAGAGTTTAACTCGTATGAGGAAACACTGCATC
TTTTAAGCAGCCCTACCAATGCCAAGCGTCTCAGACGCTCTATTGCTGATGCTAAAGCTGGTCGCTTGATTGAACGTGAG
ATTGATGCCAAAGATATGCCACTCACTTTTGATGATGAGGTAGACGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1G4U731