Detailed information of TA system TA022982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 1827650..1828300
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: PPHA_RS08495
• Protein ID: WP_012508438.1
• Coordinates: 1827650..1827931 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B4SB06
• Locus tag: PPHA_RS08500
• Protein ID: WP_012508439.1
• Coordinates: 1828073..1828300 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS08460	1822804..1823028	+	225	WP_012508431.1	DUF433 domain-containing protein	-
PPHA_RS08465	1823025..1823369	+	345	WP_012508432.1	DUF5615 family PIN-like protein	-
PPHA_RS08470	1823395..1823631	+	237	Protein_1659	hypothetical protein	-
PPHA_RS08475	1823841..1824407	+	567	WP_041526793.1	hypothetical protein	-
PPHA_RS08480	1824416..1826869	+	2454	WP_012508435.1	tetratricopeptide repeat protein	-
PPHA_RS08485	1827057..1827290	+	234	WP_041526794.1	hypothetical protein	-
PPHA_RS08490	1827457..1827669	-	213	WP_041526492.1	hypothetical protein	-
PPHA_RS08495	1827650..1827931	-	282	WP_012508438.1	BrnT family toxin	Toxin
PPHA_RS08500	1828073..1828300	-	228	WP_012508439.1	CopG family transcriptional regulator	Antitoxin
PPHA_RS08505	1828350..1828853	-	504	WP_012508440.1	GNAT family N-acetyltransferase	-
PPHA_RS08510	1828860..1829138	-	279	WP_012508441.1	DUF1778 domain-containing protein	-
PPHA_RS15115	1829228..1830236	-	1009	Protein_1668	ABC transporter permease	-
PPHA_RS08520	1830494..1830883	-	390	WP_012508442.1	type II toxin-antitoxin system VapC family toxin	-
PPHA_RS08525	1830880..1831110	-	231	WP_012508443.1	hypothetical protein	-
PPHA_RS08530	1831362..1831613	-	252	WP_012508444.1	UPF0175 family protein	-
PPHA_RS08535	1832032..1832358	+	327	WP_012508445.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPHA_RS08540	1832351..1832677	+	327	WP_012508446.1	helix-turn-helix domain-containing protein	-
PPHA_RS08545	1832737..1832918	-	182	Protein_1674	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-84)

Antitoxin

• CopG antitoxin (1-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10721.93 Da
• Isoelectric Point: 6.7600

>T22982 WP_012508438.1 NC_011060:c1827931-1827650 [Pelodictyon phaeoclathratiforme BU-1]
MLSFEWNSQKAFSNEQKHGISFDEASTVFGDTLSLTLHDPLHSENEDRFVVIGRSHKNRILTVVHTERGDILRIISARKA
TKNERLQYESHAE

Nucleotide

• Length: 282 bp

>T22982 NC_011060:c1827931-1827650 [Pelodictyon phaeoclathratiforme BU-1]
ATGCTATCGTTTGAGTGGAATAGTCAAAAGGCGTTCAGCAATGAACAAAAACATGGAATTTCTTTCGATGAGGCCAGTAC
AGTTTTTGGAGACACTCTTTCACTGACACTCCACGACCCCCTTCATTCTGAAAATGAGGATCGGTTTGTGGTCATTGGGA
GATCTCATAAAAACCGTATATTAACTGTCGTTCATACGGAGCGGGGAGATATACTTCGAATAATCAGTGCCAGAAAGGCG
ACGAAAAACGAGAGGTTACAGTATGAAAGTCATGCAGAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8723.99 Da
• Isoelectric Point: 8.0185

>AT22982 WP_012508439.1 NC_011060:c1828300-1828073 [Pelodictyon phaeoclathratiforme BU-1]
MKTEEFDKQFDDGADITNFLDLSKAKRIKQQHKRVDVDFPVRMIDSLDKEATCLGVSRQSVIKVWLGERLQQQKA

Nucleotide

• Length: 228 bp

>AT22982 NC_011060:c1828300-1828073 [Pelodictyon phaeoclathratiforme BU-1]
ATGAAAACAGAGGAATTTGACAAGCAATTTGATGATGGTGCAGATATCACCAATTTCCTTGACCTGAGCAAGGCGAAAAG
GATAAAACAACAGCACAAAAGGGTGGATGTTGATTTCCCTGTCAGGATGATTGACTCACTCGATAAAGAGGCAACCTGTC
TTGGCGTATCGCGTCAGTCTGTGATTAAAGTTTGGCTCGGAGAACGTCTTCAACAACAGAAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: B4SB06